(a)
Interpretation:
Effects on eIF-4 helicase activity in the presence of eIF-4H should be determined.
Concept introduction:
Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.
(b)
Interpretation:
The importance of measuring the helicase activity of eIF-4H as control should be determined.
Concept introduction:
Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.
(c)
Interpretation:
The ratio of eIF-4H and eIF-4 should be determined to attain optimal activity.
Concept introduction:
Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.
(d)
Interpretation:
The varying effect f eIF-4H with helix stability should be determined.
Concept introduction:
Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.
(e)
Interpretation:
The effect of eIF-4H on the helicase activity of eIF-4A should be determined.
Concept introduction:
Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.
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Chapter 31 Solutions
SAPLINGPLUS F/BIOCHEM+ICLICKER REEF-CODE
- Balance the following equation and list of coefficients in order from left to right. SF4+H2O+—-> H2SO3+HFarrow_forwardProblem 15 of 15 Submit Using the following reaction data points, construct Lineweaver-Burk plots for an enzyme with and without an inhibitor by dragging the points to their relevant coordinates on the graph and drawing a line of best fit. Using the information from this plot, determine the type of inhibitor present. 1 mM-1 1 s mM -1 [S]' V' with 10 μg per 20 54 10 36 20 5 27 2.5 23 1.25 20 Answer: |||arrow_forward12:33 CO Problem 4 of 15 4G 54% Done On the following Lineweaver-Burk -1 plot, identify the by dragging the Km point to the appropriate value. 1/V 40 35- 30- 25 20 15 10- T Км -15 10 -5 0 5 ||| 10 15 №20 25 25 30 1/[S] Г powered by desmosarrow_forward
- 1:30 5G 47% Problem 10 of 15 Submit Using the following reaction data points, construct a Lineweaver-Burk plot for an enzyme with and without a competitive inhibitor by dragging the points to their relevant coordinates on the graph and drawing a line of best fit. 1 -1 1 mM [S]' s mM¹ with 10 mg pe 20 V' 54 10 36 > ст 5 27 2.5 23 1.25 20 Answer: |||arrow_forwardProblem 14 of 15 Submit Using the following reaction data points, construct Lineweaver-Burk plots for an enzyme with and without an inhibitor by dragging the points to their relevant coordinates on the graph and drawing a line of best fit. Using the information from this plot, determine the type of inhibitor present. 1 mM-1 1 s mM -1 [S]' V' with 10 μg per 20 54 10 36 20 5 27 2.5 23 1.25 20 Answer: |||arrow_forward12:36 CO Problem 9 of 15 4G. 53% Submit Using the following reaction data points, construct a Lineweaver-Burk plot by dragging the points to their relevant coordinates on the graph and drawing a line of best fit. Based on the plot, determine the value of the catalytic efficiency (specificity constant) given that the enzyme concentration in this experiment is 5.0 μ.Μ. 1 [S] ¨‚ μM-1 1 V sμM-1 100.0 0.100 75.0 0.080 50.0 0.060 15.0 0.030 10.0 0.025 5.0 0.020 Answer: ||| O Гarrow_forward
- Problem 11 of 15 Submit Using the following reaction data points, construct a Lineweaver-Burk plot for an enzyme with and without a noncompetitive inhibitor by dragging the points to their relevant coordinates on the graph and drawing a line of best fit. 1 -1 1 mM [S]' 20 V' s mM¹ with 10 μg per 54 10 36 > ст 5 27 2.5 23 1.25 20 Answer: |||arrow_forwardProblem 13 of 15 Submit Using the following reaction data points, construct Lineweaver-Burk plots for an enzyme with and without an inhibitor by dragging the points to their relevant coordinates on the graph and drawing a line of best fit. Using the information from this plot, determine the type of inhibitor present. 1 mM-1 1 s mM -1 [S]' V' with 10 μg per 20 54 10 36 20 5 27 2.5 23 1.25 20 Answer: |||arrow_forward12:33 CO Problem 8 of 15 4G. 53% Submit Using the following reaction data points, construct a Lineweaver-Burk plot by dragging the points to their relevant coordinates on the graph and drawing a line of best fit. Based on the plot, determine the value of kcat given that the enzyme concentration in this experiment is 5.0 μM. 1 [S] , мм -1 1 V₁ s μM 1 100.0 0.100 75.0 0.080 50.0 0.060 15.0 0.030 10.0 0.025 5.0 0.020 Answer: ||| Гarrow_forward
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