MICROBIOLOGY: EVOLV.SCI.-W/ACCESS>CI<
MICROBIOLOGY: EVOLV.SCI.-W/ACCESS>CI<
4th Edition
ISBN: 9780393622805
Author: SLONCZEWSKI
Publisher: Norton custom
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Chapter 3, Problem 9RQ
Summary Introduction

To review:

The subcellular structures present in certain cells having various functions like magnetotaxis or photosynthesis.

Introduction:

The subcellular structures are the simple structures that are present inside the cell. These structures can be easily visualized by transmission electron microscope or scanning electron microscope. A few examples of subcellular structures are mitochondria, endoplasmic reticulum, Golgi apparatus, and nucleus.

In cells, there are several major structures that are involved in content organization, DNA (deoxyribonucleic acid) maintenance, and in the synthesis of new parts. In order to adapt to the diverse environment and metabolic processes, various species evolve some subcellular structures like thylakoids, storage granules, and carboxysomes. Photosynthetic bacteria or organisms are associated with the formation of their own food.

Thylakoids are membrane-bound sections that lie in cyanobacteria and chloroplasts. Photosynthetic bacteria evolve this subcellular structure to increase the collecting surface of membranes involved in photosynthesis. These structures carry out light reactions for energy storage and absorption of photons. This energy is used for carbon dioxide fixation that takes place inside the covered polyhedral bodies which are carboxysomes along with the Rubisco enzyme for fixation. Phycobilisomes are also found in association with the thylakoid membrane in order to harvest sunlight.

Storage granules are generated by nonphototrophic bacteria of soil. These granules store essential metabolites and reserved energy. Magnetotaxis is a form of chemotaxis that possesses the ability to respond toward magnetism. The magnetotactic bacteria sense magnetism and get oriented along with the magnetic field. The subcellular structure is flagella that moves clockwise and anticlockwise on the basis of the magnetism.

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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
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