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(a)
To explain: The reason for the presence of only one allele at some loci.
Concept introduction:
The
(b)
To identify: The suspect that could be a possible source of the blood sample.
Concept introduction:
The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated using restriction enzymes into single strands. Then, these strands are incubated with DNA polymerase such as Taq polymerase, two primers, and deoxynucleotide triphosphates (dNTPs). Different types of fluorescent primers are used in PCR, and the PCR-amplified products are subjected to electrophoretograms to identify the alleles.
(c)
To determine: Whether the suspect could be recognized using one of the three given STR loci.
Concept introduction:
The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated using restriction enzymes into single strands. Then, these strands are incubated with DNA polymerase such as Taq polymerase, two primers, and deoxynucleotide triphosphate (dNTPs). Different types of fluorescent primers are used in PCR, and the PCR-amplified products are subjected to electrophoretograms to identify the alleles.
(d)
To compare: The amount of DNA obtained from suspect 1 with suspect 4.
Concept introduction:
The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated using restriction enzymes into single strands. Then, these strands are incubated with DNA polymerase such as Taq polymerase, two primers, and deoxynucleotide triphosphate (dNTPs). Different types of fluorescent primers are used in PCR, and the PCR-amplified products are subjected to electrophoretograms to identify the alleles.
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Chapter 3 Solutions
Biochemistry 410/411 Textbook - 5th Edition - Custom Texas A&M University
- In a diffraction experiment of a native crystal, intensity of reflection (-1 0 6) is equivalent to the intensity of reflection (1 0 -6). true or false?arrow_forwardin an x-ray diffraction experiment, moving the detector farther away from the crystal will allow collection of reflection of reflections with high Miller indices. true or false?arrow_forwardShow the mechanism for the acid-catalyzed formation of an [α-1,6] glycosidic linkagebetween two molecules of α-D-glucopyranose.arrow_forward
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- Answerarrow_forward13. Which one is the major organic product of the following sequence of reactions? A OH (CH3)2CHCH2COOH SOCI2 CH3OH 1. CH3MgBr 2. H₂O, H+ B C D OH E OHarrow_forward14. Which one is the major organic product of the following sequence of reactions? (CH3)2CH-COCI CH3OH 1. DIBALH, -78°C 1. PhCH2MgBr ? 2. H2O, HCI 2. H2O, HCI OH OMe A Ph B Ph OH Ph C OMe Ph D E OH .Pharrow_forward
- 6. Which one is the major organic product obtained from the following reaction? CO₂Me 1. LiAlH4 2. H₂O CH₂OH CH₂OCH3 5555 HO A B HO C HO D CH₂OH E ?arrow_forward1. (10 points) Pulverized coal pellets, which may be ° approximated as carbon spheres of radius r = 1 mm, are burned in a pure oxygen atmosphere at 1450 K and 1 atm. Oxygen is transferred to the particle surface by diffusion, where it is consumed in the reaction C + O₂ →> CO₂. The reaction rate is first order and of the form No2 = k₁C₁₂(r), where k₁ = 0.1 m/s. Neglecting changes in r, determine the steady-state O₂ molar consumption rate in kmol/s. At 1450 K, the binary diffusion coefficient for O2 and CO2 is 1.71 x 10ª m²/s.arrow_forward2. (20 points) Consider combustion of hydrogen gas in a mixture of hydrogen and oxygen adjacent to the metal wall of a combustion chamber. Combustion occurs at constant temperature and pressure according to the chemical reaction 2H₂+ O₂→ 2H₂O. Measurements under steady-state conditions at 10 mm from the wall indicate that the molar concentrations of hydrogen, oxygen, and water vapor are 0.10, 0.10, and 0.20 kmol/m³, respectively. The generation rate of water vapor is 0.96x102 kmol/m³s throughout the region of interest. The binary diffusion coefficient for each of the species (H, O̟, and H₂O) in the remaining species is 0.6 X 10-5 m²/s. (a) Determine an expression for and make a qualitative plot of C as a function of distance from the wall. H2 (b) Determine the value of C2 at the wall. H2 (c) On the same coordinates used in part (a), sketch curves for the concentrations of oxygen and water vapor. This will require you to calculate Co, and C. 02 H20 (d) What is the molar flux of water…arrow_forward
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