EBK LEHNINGER PRINCIPLES OF BIOCHEMISTR
EBK LEHNINGER PRINCIPLES OF BIOCHEMISTR
7th Edition
ISBN: 8220103662253
Author: nelson
Publisher: MAC HIGHER
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Chapter 3, Problem 22P

(a)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with positively charged groups.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be separated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; anion-exchange chromatography and cation-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

(b)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with negatively charged groups.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be seprated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; cation-exchange chromatography and anion-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

(c)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in a size-exclusion chromatography column designed to separate small peptides.

Introduction:

Size-exclusion chromatography is also termed as molecular sieve chromatography. In this procedure, the molecules are separated according to their size and molecular weight. Size-exclusion chromatography is basically used for macromolecules such as proteins.

(d)

Summary Introduction

To determine: The peptide that contains ATP-binding motif shown in the given sequence logo.

Introduction:

ATP (adenosine triphosphate) is a complex organic compound that cell produces during the process of cellular respiration. Cellular respiration is the process by which energy is produced in a cell. An ATP molecule is comprised of three basic parts; adenine, ribose sugar, and three molecules of phosphate.

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