Chapter 3, Problem 9P

(a)

Summary Introduction

To calculate: The minimum molecular weight of bovine serum albumin
(BSA) assuming that there is only one Trp residue per protein molecule.

Introduction:

Molecular weight refers to the mass of a molecule. It is simply calculated as the sum of atomic masses of all the atoms present in that particular molecule.

(b)

Summary Introduction

To determine: The number of Trp residues present in one molecule of serum albumin if size-exclusion chromatography of BSA gives a molecular weight of 70,000.

Introduction:

Size exclusion chromatography is also termed as molecular sieve chromatography. In this type of chromatography, molecules are separated on the basis of their size.