EP MICROBIOLOGY:W/DISEASES BY..-MOD.ACC
5th Edition
ISBN: 9780134607894
Author: BAUMAN
Publisher: PEARSON CO
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Chapter 3, Problem 12SA
Summary Introduction
To answer:
Contrast bacterial and eukaryotic cells.
Introduction:
The unicellular organisms are separated into prokaryotic and eukaryotic organisms. Though eukaryotic organisms are multi-cellular. Some of the oldest known species of algae, protozoa, and
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 3 Solutions
EP MICROBIOLOGY:W/DISEASES BY..-MOD.ACC
Ch. 3 - Prob. 1TMWCh. 3 - In 1985, an Israeli scientist discovered the...Ch. 3 - Why is a pilus a type of fimbria, but a flagellum...Ch. 3 - Why is the microbe illustrated in Figure 3.2 more...Ch. 3 - The Big Game College sophomore Nadia is a star...Ch. 3 - When the bacterium Escherichia coli is grown in a...Ch. 3 - Prob. 6TMWCh. 3 - Why do scientists consider bacterial and archaeal...Ch. 3 - Why did scientists in the 19th and early 20th...Ch. 3 - Why do some scientists consider archaea, which are...
Ch. 3 - Why are eukaryotic glycocalyces covalently bound...Ch. 3 - Many antimicrobial drugs target bacterial cell...Ch. 3 - Colchicine is a drug that inhibits microtubule...Ch. 3 - A cell may allow a large or charged chemical to...Ch. 3 - Which of the following statements concerning...Ch. 3 - A 9 + 2 arrangement of microtubules is seen in...Ch. 3 - Which of the following is most associated with...Ch. 3 - Which of the following is not associated with...Ch. 3 - Which of the following is true of Svedbergs? a....Ch. 3 - Which of the following statements is true? a. The...Ch. 3 - Prob. 8MCCh. 3 - Bacterial flagella are ______________. a. anchored...Ch. 3 - Prob. 10MCCh. 3 - A Gram-negative cell is moving uric acid across...Ch. 3 - Gram-positive bacteria _______________. a. have a...Ch. 3 - Endospores ________________. a. are reproductive...Ch. 3 - Prob. 14MCCh. 3 - Dipicolinic acid is an important component of...Ch. 3 - Match the structures on the left with the...Ch. 3 - Match the term on the left with its description on...Ch. 3 - Label the structures of the following prokaryotic...Ch. 3 - Label each type of flagellar arrangement.Ch. 3 - A scientist who is studying passive movement of...Ch. 3 - Describe (or draw) an example of diffusion down a...Ch. 3 - Sketch, name, and describe three flagellar...Ch. 3 - Prob. 3SACh. 3 - The term fluid mosaic has been used in describing...Ch. 3 - A local newspaper writer has contacted you, an...Ch. 3 - Prob. 6SACh. 3 - Compare bacterial cells and algal cells, giving at...Ch. 3 - Contrast a cell of Streptococcus pyogenes (a...Ch. 3 - Differentiate among pili, fimbriae, and cilia,...Ch. 3 - Prob. 10SACh. 3 - Prob. 11SACh. 3 - Prob. 12SACh. 3 - What is the function of glycocalyces and fimbriae...Ch. 3 - Prob. 14SACh. 3 - Compare and contrast three types of passive...Ch. 3 - Prob. 16SACh. 3 - Prob. 17SACh. 3 - Prob. 18SACh. 3 - Prob. 1CTCh. 3 - Methylene blue binds to DNA. What structures in a...Ch. 3 - Prob. 3CTCh. 3 - Prob. 4CTCh. 3 - A researcher carefully inserts an electrode into...Ch. 3 - Prob. 6CTCh. 3 - An electron micrograph of a newly discovered cell...Ch. 3 - An entry in a recent scientific journal reports...Ch. 3 - Prob. 9CTCh. 3 - Prob. 10CTCh. 3 - Prob. 11CTCh. 3 - Prob. 12CTCh. 3 - Prob. 13CTCh. 3 - Prob. 14CTCh. 3 - Using the following terms, fill in the following...
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have shipped your samples off for sequencing and are now waiting for the results. Out of curiosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidase enzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you do see a color change to purple during the Oxidase test. What results can you conclude from this? O Catalase-/ Oxidase + O Catalase +/ Oxidase + Catalase + / Oxidase- O Catalase / Oxidase - O None of the abovearrow_forwardWhich of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forwardWhy are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forward
- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
- Which of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forwardWhat is the most appropriate purpose of building a phylogenetic tree? They look awesome You can use a tree to compare morphological characteristics of organisms It can be used to establish and analyze evolutionary relationships between species All of the abovearrow_forward
- Which of the following sequencing techniques can identify down to the strain level? O Multilocus sequence typing Genomic fingerprinting Whole genome sequencing OSNP analysis All of the abovearrow_forwardWhat is the "gold standard" that is currently applied to species designations in microbiology? 97% between species: 50% among whole genome 90% between species: 75% among whole genome 99% between species; 97% among whole genome 97% between species: 70% among whole genome Onone of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have decided to send your sample off for sequencing. You need to determine which type of sequencing to use for the preliminary identification of your species. You decide that, for now, you only need to be able to identify the family and genus levels. Which type of sequencing do you think is the most appropriate? O Genomic Fingerprinting O Whole Genome Sequencing O 16S rDNA Sequencing O DNA-DNA hybridization Nextarrow_forward
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