Biochemistry, The Molecular Basis of Life, 6th Edition
6th Edition
ISBN: 9780190259204
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 3, Problem 10RQ
Summary Introduction
Interpretation:
Determine the directionin which the water flows when a dialysis bag containing 3 M sugar fructose solution isplaced in each of the givensolutions.
1 M sodium lactate
3M sodium lactate
4.5 M sodium lactate
Concept introduction:
The water movement from low concentration side to high concentration sideis known as osmosis whereas the movement of solute particles takes place from high concentration side to low concentration side is known as diffusion.
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The preferred substrate is T because the enzyme half-saturates at 7.00 mM for T, but requires 28.0 mM for U, and 112 mM for S.
b
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The rate constant k 2 with substrate S is 9.60×107 sec-1, with substrate T, k 2 = 6.00×104 sec-1, and with substrate U, k 2 = 2.40×106 sec-1.
Calculate the catalytic efficiency with S, T, and U.
Catalytic efficiency with S =
Catalytic efficiency with T =
Catalytic efficiency with U =
Does enzyme A use substrate S, substrate T, or substrate U with greater catalytic efficiency?
Fumerase catalyzes the conversion of fumerate to malate.
fumerate + H2O ⇋ malate
The turnover number, kcat, for fumerase is 8.00×102 sec-1. The Km of this enzyme for fumerate is 5.00×10-3 μmol mL-1.
a
In an experiment using 2.00×10-3 μmol·mL-1, what is Vmax?
Suppose you wanted to make a buffer of exactly pH 7.00 using KH2PO4 and Na2HPO4. If the final solution was 0.18 M in KH2PO4, you would need 0.25 M Na2HPO4. Now assume you wish to make a buffer at the same pH, using the same substances, but want the total phosphate molarity ([HPO42−]+[H2PO−4]) to equal 0.20 M. What concentration of the Na2HPO4 would be required?
Chapter 3 Solutions
Biochemistry, The Molecular Basis of Life, 6th Edition
Ch. 3 - Prob. 1QCh. 3 - Prob. 2QCh. 3 - Prob. 3QCh. 3 - Prob. 4QCh. 3 - Prob. 1RQCh. 3 - Prob. 2RQCh. 3 - Prob. 3RQCh. 3 - Prob. 4RQCh. 3 - Prob. 5RQCh. 3 - Prob. 6RQ
Ch. 3 - Prob. 7RQCh. 3 - Prob. 8RQCh. 3 - Prob. 9RQCh. 3 - Prob. 10RQCh. 3 - Prob. 11RQCh. 3 - Prob. 12RQCh. 3 - Prob. 13RQCh. 3 - Prob. 14RQCh. 3 - Prob. 15RQCh. 3 - Prob. 16RQCh. 3 - Prob. 17RQCh. 3 - Prob. 18RQCh. 3 - Prob. 19RQCh. 3 - Prob. 20RQCh. 3 - Prob. 21RQCh. 3 - Prob. 22RQCh. 3 - Prob. 23RQCh. 3 - Prob. 24RQCh. 3 - Prob. 25RQCh. 3 - Prob. 26RQCh. 3 - Prob. 27RQCh. 3 - Prob. 28RQCh. 3 - Prob. 29RQCh. 3 - Prob. 30RQCh. 3 - Prob. 31RQCh. 3 - Prob. 32RQCh. 3 - Prob. 33RQCh. 3 - Prob. 34RQCh. 3 - Prob. 35RQCh. 3 - Prob. 36FBCh. 3 - Prob. 37FBCh. 3 - Prob. 38FBCh. 3 - Prob. 39FBCh. 3 - Prob. 40FBCh. 3 - Prob. 41FBCh. 3 - Prob. 42FBCh. 3 - Prob. 43FBCh. 3 - Prob. 44FBCh. 3 - Prob. 45FBCh. 3 - Prob. 46SACh. 3 - Prob. 47SACh. 3 - Prob. 48SACh. 3 - Prob. 49SACh. 3 - Prob. 50SACh. 3 - Prob. 51TQCh. 3 - Prob. 52TQCh. 3 - Prob. 53TQCh. 3 - Prob. 54TQCh. 3 - Prob. 55TQCh. 3 - Prob. 56TQCh. 3 - Prob. 57TQCh. 3 - Prob. 58TQCh. 3 - Prob. 59TQCh. 3 - Prob. 60TQCh. 3 - Prob. 61TQCh. 3 - Prob. 62TQCh. 3 - Prob. 63TQCh. 3 - Prob. 64TQCh. 3 - Prob. 65TQCh. 3 - Prob. 66TQCh. 3 - Prob. 67TQCh. 3 - Prob. 68TQCh. 3 - Prob. 69TQCh. 3 - Prob. 70TQCh. 3 - Prob. 71TQCh. 3 - Prob. 72TQCh. 3 - Prob. 73TQCh. 3 - Prob. 74TQCh. 3 - Prob. 75TQCh. 3 - Prob. 76TQ
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