Principles of Instrumental Analysis
7th Edition
ISBN: 9781305577213
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cengage Learning
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Question
Chapter 29, Problem 29.2QAP
Interpretation Introduction
Interpretation:
The properties of a supercritical fluid that are important in chromatography are to be discussed.
Concept introduction:
When there is a use of supercritical fluid like carbon dioxide in mobile phase of chromatography, it is called as supercritical fluid chromatography.
The supercritical fluid contains density and diffusivity characteristics in between liquid and gas forms but the viscosity is similar to the gaseous form.
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PAPER CROMATOGRAPHY
• Define chromatography
• Demonstrate an understanding of the process of
chromatography
• Describe the steps involved in a chromatography
investigation
Explanation
Coloured inks are often made up of several different inks.
Each colour of ink will get carried a different distance by the
water, allowing you to see all of the different inks. Try this with
different colours to see what actually goes into a colour. This
technique is called chromatography and can be used in
chemistry to find out what is in an unknown mixture.
II. Materials
.Felt tip pens (non-washable)
. Kitchen roll
.A bowl or cup
. Water
• Scissors
III. Health and safety
• Make sure that you thoroughly clean any equipment after the
experiment before using for food
IV. Procedure
Method 1
1. Take a sheet of kitchen roll and draw a simple picture with a
non-washable felt tip pen.
2. Place the sheet over an empty bowl. This is to keep the paper
from getting too wet.
3. Carefully add a few drops of water…
Can paper chromatography be used to separate and identify very volatile substances? Why? Please explain comprehensively.
Consider four distinct physical properties: boiling point, melting point, solubility, and density. Which of these physical properties do you think allows thin layer chromatography (TLC) to work? Choose one then, explain your reasoning.
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- Use a chemical dictionary, chemical text or encyclopedia to find a specific definition of "chromatography." List a few mobile and stationary phases.arrow_forwardThe paper used in chromatography is made of molecules with___parts A polar B nonpolararrow_forwardWhat are prominent applications of High Performance Liquid Chromatography?arrow_forward
- Can paper chromatography be used to separate and identify very volatile substances? How do I prove this.arrow_forwardExplain the following Chromatography (for drug analysis) Thin-layer chromatography (TLC) Gas chromatography (GC) Liquid chromatography (LC) (i.e, high performance liquid chromatography or HPLC)arrow_forwardGas Chromatography (GC) is one of the useful techniques in analytical chemistry. Give two important components in GC.arrow_forward
- Describe chromatography. Include the following words: Physical change, Intermolecular Forces, Mixture, Solute, Solvent.arrow_forwardWhat kind of mixture of substance has Distillation and Chromatography has?arrow_forwardChemistry Consider the following chromatogram. The early eluting peaks and later eluting peaks exhibit a problem. Propose a way in gas chromatography to eliminate both problems.arrow_forward
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