Pearson eText Microbiology: An Introduction -- Instant Access (Pearson+)
Pearson eText Microbiology: An Introduction -- Instant Access (Pearson+)
13th Edition
ISBN: 9780135789377
Author: Gerard Tortora, Berdell Funke
Publisher: PEARSON+
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Chapter 28, Problem 3CAE

The antibiotic efrotomycin is produced by Streptomyces lactamdurans. S. lactamdurans was grown in 40,000 liters of medium. The medium consisted of glucose, maltose, soybean oil, (NH4)2SO4, NaCl, KH2PO4, and Na2HPO4. The culture was aerated and maintained at 28°C. The following results were obtained from analyses of the culture medium during cell growth:

Chapter 28, Problem 3CAE, The antibiotic efrotomycin is produced by Streptomyces lactamdurans. S. lactamdurans was grown in , example  1

Chapter 28, Problem 3CAE, The antibiotic efrotomycin is produced by Streptomyces lactamdurans. S. lactamdurans was grown in , example  2

  1. a. Under what conditions is the most efrotomycin produced? Is it a primary or secondary metabolite?
  2. b. Which is used first, maltose or glucose? Suggest a reason for this.
  3. c. What is the purpose of each ingredient in the growth medium? (Hint: See Chapter 6.)
  4. d. What is Streptomyces? (Hint: See Chapter 11.)
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After you feel comfortable with your counting method and identifying cells in the various stages of mitosis, use the four images below of whitefish blastula to count the cells in each stage until you reach 100 total cells, recording your data below in Data Table 1. (You may not need to use all four images. Stop counting when you reach 100 total cells.) After totaling the cells in each stage, calculate the percent of cells in each stage. (Divide total of stage by overall total of 100 and then multiply by 100 to obtain percentage.)   Data Table 1Stage    Totals    PercentInterphase        Mitosis:        Prophase        Metaphase        Anaphase        Telophase        Cytokinesis        Totals    100    100% To find the length of time whitefish blastula cells spend in each stage, multiply the percent (recorded as a decimal, in other words take the percent number and divide by 100) by 24 hours. (Example: If percent is 20%, then Time in Hours = .2 * 24 = 4.8) Record your data in Data…

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Pearson eText Microbiology: An Introduction -- Instant Access (Pearson+)

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