Chapter 27.4, Problem 27.3P

Interpretation Introduction

Interpretation:

A restriction enzyme known as Bg/II cuts after Adenine near to $5\text{'}$ in the given DNA. The complementary ${\text{3}}^{\text{'}}{\text{ to 5}}^{\text{'}}$ strand should be drawn for the above DNA and shown where it is cut by the same enzyme.

Concept Introduction:

• Restriction endonuclease is a restriction enzyme that cuts double stranded or single stranded DNA at specific recognition nucleotide sequences (mostly palindromic) known as restriction sites.
• Palindromic sequence is the mirror like palindrome in which the same forward and backwards are on a single strand of DNA strand.

  Example for palindrome sequence:

  $\begin{array}{l}5\text{'}-G-T-T-A-A-C-3\text{'}\\ 3\text{'}-C-A-A-T-T-G-5\text{'}\end{array}$

• The base sequences that most restriction endonuclease recognizes are palindromes.