Explanation Reason for correct option: Option (a) is muscle. Muscle cells need glucose for the respiration purpose. So, insulin, with the help of GLUT, transport glucose inside the muscle cells. Hence, this option is correct. Option (c) is adipose tissue...

9th Edition

ISBN: 9781319402877

Author: BERG

Publisher: MAC HIGHER

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Question

Chapter 27, Problem 2P

Interpretation Introduction

Interpretation:

The tissues that are primary targets of the insulin should be determined.

Concept introduction:

Insulin is a type of anabolic hormone which is produced and secreted by islets cells of the pancreas. The main function of insulin is to control the blood glucose level in the body. It prevents the high glucose level (hyperglycemia) and low blood glucose level (hypoglycemia).

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Chapter 27, Problem 1P

Chapter 27, Problem 3P

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Chapter 27 Solutions

BIOCHEMISTRY 2 TERM ACCESS

Ch. 27 - Prob. 1P Ch. 27 - Prob. 2P Ch. 27 - Prob. 3P Ch. 27 - Prob. 4P Ch. 27 - Prob. 5P Ch. 27 - Prob. 6P Ch. 27 - Prob. 7P Ch. 27 - Prob. 8P Ch. 27 - Prob. 9P Ch. 27 - Prob. 10P

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Solution Summary: The author explains that insulin is a type of anabolic hormone produced and secreted by islets cells of the pancreas.

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