Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
2nd Edition
ISBN: 9780134702339
Author: Erin C. Amerman
Publisher: PEARSON
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Chapter 25.3, Problem 10QC
Summary Introduction
To review:
The role of chloride and magnesium ions in the physiology of humans.
Introduction:
Electrolytes are compounds, which have the ability to ionize in the solution. In the human body, electrolytes are salts, which can dissociate into ions of sodium, chlorine, magnesium, calcium, potassium, etc. The systems that maintain electrolyte balance are endocrine system and urinary system.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 25 Solutions
Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
Ch. 25.1 - 1. What is a body fluid?
Ch. 25.1 - 2. What is balance with respect to body fluids?
Ch. 25.1 - How does an electrolyte differ from a...Ch. 25.1 - What is electrolyte balance?Ch. 25.1 - 5. How do acids and bases differ?
Ch. 25.1 - 6. Which pH values are acidic, basic, and...Ch. 25.2 - Prob. 1QCCh. 25.2 - What factors affect total body water?Ch. 25.2 - 3. Where are the intracellular and extracellular...Ch. 25.2 - Prob. 4QC
Ch. 25.2 - Prob. 5QCCh. 25.2 - Prob. 6QCCh. 25.2 - Prob. 7QCCh. 25.2 - How is thirst stimulated?Ch. 25.2 - How are fluids lost from the body?Ch. 25.2 - 10. What are the water requirements for an...Ch. 25.2 - 11. What is the role of ADH in fluid balance?
Ch. 25.2 - How is ADH secretion stimulated?Ch. 25.2 - How does dehydration affect the volume of the...Ch. 25.2 - Prob. 14QCCh. 25.2 - 15. How do dehydration and overhydration differ...Ch. 25.3 - What are the main roles of sodium ions in the...Ch. 25.3 - How is sodium ion concentration regulated?Ch. 25.3 - Prob. 3QCCh. 25.3 - Prob. 4QCCh. 25.3 - 5. How is the concentration of potassium ions in...Ch. 25.3 - 6. What happens to the resting membrane potential...Ch. 25.3 - Prob. 7QCCh. 25.3 - Prob. 8QCCh. 25.3 - Prob. 9QCCh. 25.3 - Prob. 10QCCh. 25.3 - 11. How is chloride ion reabsorption in the...Ch. 25.3 - 12. How is the concentration of magnesium ions in...Ch. 25.4 - What are the major sources of acids for the body?Ch. 25.4 - Prob. 2QCCh. 25.4 - Prob. 3QCCh. 25.4 - Prob. 4QCCh. 25.4 - Prob. 5QCCh. 25.4 - Prob. 6QCCh. 25.4 - Prob. 7QCCh. 25.4 - Prob. 8QCCh. 25.4 - How do metabolic acidosis and respiratory acidosis...Ch. 25.4 - Prob. 10QCCh. 25.4 - Prob. 11QCCh. 25.5 - Prob. 1QCCh. 25.5 - Prob. 2QCCh. 25 - Prob. 1CYRCh. 25 - 2. How does an electrolyte differ from a...Ch. 25 - Prob. 3CYRCh. 25 - Prob. 4CYRCh. 25 - Prob. 5CYRCh. 25 - Prob. 6CYRCh. 25 - Which of the following is false with respect to...Ch. 25 - Prob. 8CYRCh. 25 - Prob. 9CYRCh. 25 - Prob. 10CYRCh. 25 - Prob. 11CYRCh. 25 - Prob. 12CYRCh. 25 - Prob. 13CYRCh. 25 - Prob. 14CYRCh. 25 - 15. Which of the following mechanisms is/are used...Ch. 25 - Mark the following statements as true or false. If...Ch. 25 - Prob. 17CYRCh. 25 - 18. How does angiotensin-II help to restore fluid...Ch. 25 - Prob. 1CYUCh. 25 - Prob. 2CYUCh. 25 - Prob. 3CYUCh. 25 - Prob. 4CYUCh. 25 - Prob. 1AYKACh. 25 - Prob. 2AYKACh. 25 - Prob. 3AYKACh. 25 - Prob. 4AYKACh. 25 - Prob. 5AYKB
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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