The results obtained in Meselson and Stahl’s experiment are different from the results obtained in dispersive method of replication. Introduction : Meselson and Stahl experiment is a semi-conservative method of replication. In this the leading strand initiates the synthesis of the lagging strand. These experiments are analyzed by labeling the leading and lagging strand with radioactive atoms like N14-N15. Dispersive model is the conservative model in which many pieces of DNA are formed which form the new strands in the next generation.

Question

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Answer 1

Question

Chapter 25, Problem 1P

Summary Introduction

To explain: The results obtained in Meselson and Stahl’s experiment are different from the results obtained in dispersive method of replication.

Introduction:

Meselson and Stahl experiment is a semi-conservative method of replication. In this the leading strand initiates the synthesis of the lagging strand. These experiments are analyzed by labeling the leading and lagging strand with radioactive atoms like N14-N15. Dispersive model is the conservative model in which many pieces of DNA are formed which form the new strands in the next generation.

Expert Solution & Answer

Explanation of Solution

Explanation:

Refer to Figure 25-2 “Meselson-Stahl experiment” in the textbook. In the semi-conservative model of replication of Meselson-Stahl, labeling is done with radioactive N14-N15 in the double strand of DNA. The first generations of parents have hybrid radioactive bands. Second generation forms both N14-N15 bands in which N14 forms light colored bands and N15 forms dark colored bands. In dispersive model all the generations form dark colored bands in the second generation. Thus, presence of two bands in the Meselson-Stahl experiment ruled out the dispersive model.

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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD. The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?

Answer 2

Question

Chapter 25, Problem 1P

Summary Introduction

To explain: The results obtained in Meselson and Stahl’s experiment are different from the results obtained in dispersive method of replication.

Introduction:

Meselson and Stahl experiment is a semi-conservative method of replication. In this the leading strand initiates the synthesis of the lagging strand. These experiments are analyzed by labeling the leading and lagging strand with radioactive atoms like N14-N15. Dispersive model is the conservative model in which many pieces of DNA are formed which form the new strands in the next generation.

Expert Solution & Answer

Explanation of Solution

Explanation:

Refer to Figure 25-2 “Meselson-Stahl experiment” in the textbook. In the semi-conservative model of replication of Meselson-Stahl, labeling is done with radioactive N14-N15 in the double strand of DNA. The first generations of parents have hybrid radioactive bands. Second generation forms both N14-N15 bands in which N14 forms light colored bands and N15 forms dark colored bands. In dispersive model all the generations form dark colored bands in the second generation. Thus, presence of two bands in the Meselson-Stahl experiment ruled out the dispersive model.

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Answer 3

Question

Chapter 25, Problem 1P

Summary Introduction

To explain: The results obtained in Meselson and Stahl’s experiment are different from the results obtained in dispersive method of replication.

Introduction:

Meselson and Stahl experiment is a semi-conservative method of replication. In this the leading strand initiates the synthesis of the lagging strand. These experiments are analyzed by labeling the leading and lagging strand with radioactive atoms like N14-N15. Dispersive model is the conservative model in which many pieces of DNA are formed which form the new strands in the next generation.

Expert Solution & Answer

Explanation of Solution

Explanation:

Refer to Figure 25-2 “Meselson-Stahl experiment” in the textbook. In the semi-conservative model of replication of Meselson-Stahl, labeling is done with radioactive N14-N15 in the double strand of DNA. The first generations of parents have hybrid radioactive bands. Second generation forms both N14-N15 bands in which N14 forms light colored bands and N15 forms dark colored bands. In dispersive model all the generations form dark colored bands in the second generation. Thus, presence of two bands in the Meselson-Stahl experiment ruled out the dispersive model.

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Answer 4

Question

Chapter 25, Problem 1P

Summary Introduction

To explain: The results obtained in Meselson and Stahl’s experiment are different from the results obtained in dispersive method of replication.

Introduction:

Meselson and Stahl experiment is a semi-conservative method of replication. In this the leading strand initiates the synthesis of the lagging strand. These experiments are analyzed by labeling the leading and lagging strand with radioactive atoms like N14-N15. Dispersive model is the conservative model in which many pieces of DNA are formed which form the new strands in the next generation.

Expert Solution & Answer

Explanation of Solution

Explanation:

Refer to Figure 25-2 “Meselson-Stahl experiment” in the textbook. In the semi-conservative model of replication of Meselson-Stahl, labeling is done with radioactive N14-N15 in the double strand of DNA. The first generations of parents have hybrid radioactive bands. Second generation forms both N14-N15 bands in which N14 forms light colored bands and N15 forms dark colored bands. In dispersive model all the generations form dark colored bands in the second generation. Thus, presence of two bands in the Meselson-Stahl experiment ruled out the dispersive model.

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Answer 5

Chapter 25, Problem 1P

Summary Introduction

To explain: The results obtained in Meselson and Stahl’s experiment are different from the results obtained in dispersive method of replication.

Introduction:

Meselson and Stahl experiment is a semi-conservative method of replication. In this the leading strand initiates the synthesis of the lagging strand. These experiments are analyzed by labeling the leading and lagging strand with radioactive atoms like N14-N15. Dispersive model is the conservative model in which many pieces of DNA are formed which form the new strands in the next generation.

Expert Solution & Answer

Explanation of Solution

Explanation:

Refer to Figure 25-2 “Meselson-Stahl experiment” in the textbook. In the semi-conservative model of replication of Meselson-Stahl, labeling is done with radioactive N14-N15 in the double strand of DNA. The first generations of parents have hybrid radioactive bands. Second generation forms both N14-N15 bands in which N14 forms light colored bands and N15 forms dark colored bands. In dispersive model all the generations form dark colored bands in the second generation. Thus, presence of two bands in the Meselson-Stahl experiment ruled out the dispersive model.

Answer 6

Chapter 25, Problem 1P

Summary Introduction

To explain: The results obtained in Meselson and Stahl’s experiment are different from the results obtained in dispersive method of replication.

Introduction:

Meselson and Stahl experiment is a semi-conservative method of replication. In this the leading strand initiates the synthesis of the lagging strand. These experiments are analyzed by labeling the leading and lagging strand with radioactive atoms like N14-N15. Dispersive model is the conservative model in which many pieces of DNA are formed which form the new strands in the next generation.

Expert Solution & Answer

Explanation of Solution

Explanation:

Refer to Figure 25-2 “Meselson-Stahl experiment” in the textbook. In the semi-conservative model of replication of Meselson-Stahl, labeling is done with radioactive N14-N15 in the double strand of DNA. The first generations of parents have hybrid radioactive bands. Second generation forms both N14-N15 bands in which N14 forms light colored bands and N15 forms dark colored bands. In dispersive model all the generations form dark colored bands in the second generation. Thus, presence of two bands in the Meselson-Stahl experiment ruled out the dispersive model.

Answer 7

Chapter 25, Problem 1P

Summary Introduction

To explain: The results obtained in Meselson and Stahl’s experiment are different from the results obtained in dispersive method of replication.

Introduction:

Meselson and Stahl experiment is a semi-conservative method of replication. In this the leading strand initiates the synthesis of the lagging strand. These experiments are analyzed by labeling the leading and lagging strand with radioactive atoms like N14-N15. Dispersive model is the conservative model in which many pieces of DNA are formed which form the new strands in the next generation.

Answer 8

Expert Solution & Answer

Explanation of Solution

Explanation:

Refer to Figure 25-2 “Meselson-Stahl experiment” in the textbook. In the semi-conservative model of replication of Meselson-Stahl, labeling is done with radioactive N14-N15 in the double strand of DNA. The first generations of parents have hybrid radioactive bands. Second generation forms both N14-N15 bands in which N14 forms light colored bands and N15 forms dark colored bands. In dispersive model all the generations form dark colored bands in the second generation. Thus, presence of two bands in the Meselson-Stahl experiment ruled out the dispersive model.