Campbell Biology in Focus
3rd Edition
ISBN: 9780134710679
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Rebecca Orr
Publisher: PEARSON
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Chapter 24.3, Problem 4CC
WHAT IF? If a nonpathogenic bacterium were to acquire resistance to antibiotics, could this strain pose a health risk to people? In general, how does DNA transfer among bacteria affect the spread of resistance genes?
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 24 Solutions
Campbell Biology in Focus
Ch. 24.1 - What hypothesis did Miller test in his classic...Ch. 24.1 - How would the appearance of protocells have...Ch. 24.1 - Prob. 3CCCh. 24.1 - Prob. 4CCCh. 24.2 - Contrast the cellular and DNA structures of...Ch. 24.2 - Distinguish between the four major modes of...Ch. 24.2 - Prob. 3CCCh. 24.2 - Prob. 4CCCh. 24.3 - Although rare on a per gene basis, new mutations...Ch. 24.3 - Distinguish between the three mechanisms by which...
Ch. 24.3 - In a rapidly changing environment, which bacterial...Ch. 24.3 - WHAT IF? If a nonpathogenic bacterium were to...Ch. 24.4 - Explain how molecular systematics and metagenomics...Ch. 24.4 - WHAT IF? What would the discovery of a bacterial...Ch. 24.5 - Explain how prokaryotes, though small, can be...Ch. 24.5 - Prob. 2CCCh. 24.5 - Prob. 3CCCh. 24.5 - Prob. 4CCCh. 24 - Which of the following steps has not yet been...Ch. 24 - Fossilized stromatolites A. more than 2.8 billion...Ch. 24 - Prob. 3TYUCh. 24 - Photoautotrophs use A. light as an energy source...Ch. 24 - Which of the following statements is not true? A....Ch. 24 - Prob. 6TYUCh. 24 - Prob. 7TYUCh. 24 - SCIENTIFIC INQUIRY INTERPRET THE DATA The...Ch. 24 - FOCUS ON EVOLUTION In patients infected with...Ch. 24 - FOCUS ON ENERGY AND MATTER In a short essay (about...Ch. 24 - Prob. 11TYU
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