Campbell Biology: Custom Edition
18th Edition
ISBN: 9781323717271
Author: Urry, Cain, Wasserman, Minorsky, Reece
Publisher: PEARSON C
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Textbook Question
Chapter 24.3, Problem 2CC
WHAT IF? Ø Consider two species that diverged while geographically separated but resumed contact before reproductive isolation was complete. Predict the outcome over time if the two species mated indiscriminately and (a) hybrid offspring survived and reproduced more poorly than offspring from intraspecific matings or (b) hybrid offspring survived and reproduced as well as offspring from intraspecific matings.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 24 Solutions
Campbell Biology: Custom Edition
Ch. 24.1 - Prob. 1CCCh. 24.1 - WHAT IF? Suppose two bird species live in a...Ch. 24.2 - Summarize key differences between allopatric and...Ch. 24.2 - Prob. 2CCCh. 24.2 - WHAT IF? Is allopatric speciation more likely to...Ch. 24.2 - MAKE CONNECTIONS Review the process of meiosis in...Ch. 24.3 - 1. What are hybrid zones, and why can they be...Ch. 24.3 - WHAT IF? Consider two species that diverged while...Ch. 24.4 - Speciation can occur rapidly between diverging...Ch. 24.4 - Prob. 2CC
Ch. 24.4 - MAKE CONNECTIONS Compare Figure 13.12 with Figure...Ch. 24 - Explain the role of gene flow in the biological...Ch. 24 - Can factors that cause sympatric speciation also...Ch. 24 - What factors can support the long-term stability...Ch. 24 - Is speciation something that happened only in the...Ch. 24 - The largest unit within which gene flow can...Ch. 24 - Prob. 2TYUCh. 24 - Prob. 3TYUCh. 24 - Prob. 4TYUCh. 24 - Which of the following factors would not...Ch. 24 - Plant species A has a diploid chromosome number of...Ch. 24 - Prob. 7TYUCh. 24 - SCIENTIFIC INQUIRY DRAW IT In this chapter, you...Ch. 24 - WRITE ABOUT A THEME: INFORMATION In sexually...Ch. 24 - Prob. 10TYU
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