The sequence of amino acids encoded by the DNA sequence TTG TGC TTT has to be determined. Concept introduction: The nucelotide triplet that encodes an amino acids is called codon. Each group of three nucleotides encodes one amino acid. There are 20 amino acid present which are encoded by differnet codon. DNA sequence is always synthesized in 5 ' to 3 ' direction. The information present in DNA is transcribe into mRNA then translated by tRNA into desired sequence. The rule of base pairing are: A purine base Adenine (A) always pair with pyrimidine base Thymine (T) and the pyrimidine base Cytosine (C) always pair with purine base Guanine (G) . The complementary strand of DNA synthesized in 3 ' to 5 ' direction.

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There is an instrument in Johnson 334 that measures total-reflectance x-ray fluorescence (TXRF) to do elemental analysis (i.e., determine what elements are present in a sample). A researcher is preparing a to measure calcium content in a series of well water samples by TXRF with an internal standard of vanadium (atomic symbol: V). She has prepared a series of standard solutions to ensure a linear instrument response over the expected Ca concentration range of 40-80 ppm. The concentrations of Ca and V (ppm) and the instrument response (peak area, arbitrary units) are shown below. Also included is a sample spectrum. Equation 1 describes the response factor, K, relating the analyte signal (SA) and the standard signal (SIS) to their respective concentrations (CA and CIS). Ca, ppm V, ppm SCa, arb. units SV, arb. units 20.0 10.0 14375.11 14261.02 40.0 10.0 36182.15 17997.10 60.0 10.0 39275.74 12988.01 80.0 10.0 57530.75 14268.54 100.0…

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Chapter 24.3, Problem 24.3CYU

Interpretation Introduction

Interpretation:

The sequence of amino acids encoded by the DNA sequence TTG TGC TTT has to be determined.

Concept introduction: The nucelotide triplet that encodes an amino acids is called codon. Each group of three nucleotides encodes one amino acid. There are 20 amino acid present which are encoded by differnet codon. DNA sequence is always synthesized in 5' to 3' direction. The information present in DNA is transcribe into mRNA then translated by tRNA into desired sequence.

The rule of base pairing are: A purine base Adenine (A) always pair with pyrimidine base Thymine (T) and the pyrimidine base Cytosine (C) always pair with purine base Guanine (G). The complementary strand of DNA synthesized in 3' to 5' direction.

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There is an instrument in Johnson 334 that measures total-reflectance x-ray fluorescence (TXRF) to do elemental analysis (i.e., determine what elements are present in a sample). A researcher is preparing a to measure calcium content in a series of well water samples by TXRF with an internal standard of vanadium (atomic symbol: V). She has prepared a series of standard solutions to ensure a linear instrument response over the expected Ca concentration range of 40-80 ppm. The concentrations of Ca and V (ppm) and the instrument response (peak area, arbitrary units) are shown below. Also included is a sample spectrum. Equation 1 describes the response factor, K, relating the analyte signal (SA) and the standard signal (SIS) to their respective concentrations (CA and CIS). Ca, ppm V, ppm SCa, arb. units SV, arb. units 20.0 10.0 14375.11 14261.02 40.0 10.0 36182.15 17997.10 60.0 10.0 39275.74 12988.01 80.0 10.0 57530.75 14268.54 100.0…

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