BIOCHEMISTRY
BIOCHEMISTRY
9th Edition
ISBN: 2818440090622
Author: BERG
Publisher: MAC HIGHER
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Chapter 24, Problem 31P
Interpretation Introduction

Interpretation:

Mechanism for the interconversion of L-serine and D-serine. The equilibrium is constant for this process.

Concept introduction:

Serine racemase is an enzyme that catalyzes the interconversion of L-serine and D-serine. Therefore, this enzyme has only one substrate and one product. D-serine is a neurotransmitter that activates the NMDA receptors present in the brain. Serine racemase enzyme is coded by SRR gene. Moreover, this enzyme is PLP dependent enzyme, that catalyzes the racemization reaction and dehydration reaction.

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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD.  The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?
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