How does the metal ion in carboxypeptidase A increase the enzyme’s catalytic activity

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Answer 1

Textbook Question

Chapter 24, Problem 1P

How does the metal ion in carboxypeptidase A increase the enzyme’s catalytic activity

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The metal ion in carboxypetidase A increases the catalytic activity of the enzyme; this has to be explained.

Explanation of Solution

Carboxypetidase A is a digestive enzyme. It hydrolyzes the carboxyl terminal peptide bond and it prefers bulky and aliphatic residues.

Proposed mechanism for the catalytic reaction of carboxypetidase is given in figure 1.

Organic Chemistry, Chapter 24, Problem 1P

Figure 1

The metal ion present in the carboxypeptidase A enzyme is Zinc metal.

This metal ion (Zn2+) makes the carbonyl (C=O) carbon more susceptible to nucleophilic addition, increases the nucleophilicity of water molecule by making it like a hydroxide ion, and the negative charge of the transition state is stabilized by this metal ion.

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Predict the major products of this organic reaction: H OH 1. LiAlH4 2. H₂O ? Note: be sure you use dash and wedge bonds when necessary, for example to distinguish between major products with different stereochemistry. Click and drag to start drawing a structure. G C टे

For each reaction below, decide if the first stable organic product that forms in solution will create a new C-C bond, and check the appropriate box. Next, for each reaction to which you answered "Yes" to in the table, draw this product in the drawing area below. Note for advanced students: for this problem, don't worry if you think this product will continue to react under the current conditions - just focus on the first stable product you expect to form in solution. NH2 CI MgCl ? Will the first product that forms in this reaction create a new CC bond? Yes No MgBr ? Will the first product that forms in this reaction create a new CC bond? Yes No G टे

For each reaction below, decide if the first stable organic product that forms in solution will create a new CC bond, and check the appropriate box. Next, for each reaction to which you answered "Yes" to in the table, draw this product in the drawing area below. Note for advanced students: for this problem, don't worry if you think this product will continue to react under the current conditions - just focus on the first stable product you expect to form in solution. དྲ。 ✗MgBr ? O CI Will the first product that forms in this reaction create a new C-C bond? Yes No • ? Will the first product that forms in this reaction create a new CC bond? Yes No × : ☐ X

Answer 2

Textbook Question

Chapter 24, Problem 1P

How does the metal ion in carboxypeptidase A increase the enzyme’s catalytic activity

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The metal ion in carboxypetidase A increases the catalytic activity of the enzyme; this has to be explained.

Explanation of Solution

Carboxypetidase A is a digestive enzyme. It hydrolyzes the carboxyl terminal peptide bond and it prefers bulky and aliphatic residues.

Proposed mechanism for the catalytic reaction of carboxypetidase is given in figure 1.

Organic Chemistry, Chapter 24, Problem 1P

Figure 1

The metal ion present in the carboxypeptidase A enzyme is Zinc metal.

This metal ion (Zn2+) makes the carbonyl (C=O) carbon more susceptible to nucleophilic addition, increases the nucleophilicity of water molecule by making it like a hydroxide ion, and the negative charge of the transition state is stabilized by this metal ion.

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Answer 3

Textbook Question

Chapter 24, Problem 1P

How does the metal ion in carboxypeptidase A increase the enzyme’s catalytic activity

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The metal ion in carboxypetidase A increases the catalytic activity of the enzyme; this has to be explained.

Explanation of Solution

Carboxypetidase A is a digestive enzyme. It hydrolyzes the carboxyl terminal peptide bond and it prefers bulky and aliphatic residues.

Proposed mechanism for the catalytic reaction of carboxypetidase is given in figure 1.

Organic Chemistry, Chapter 24, Problem 1P

Figure 1

The metal ion present in the carboxypeptidase A enzyme is Zinc metal.

This metal ion (Zn2+) makes the carbonyl (C=O) carbon more susceptible to nucleophilic addition, increases the nucleophilicity of water molecule by making it like a hydroxide ion, and the negative charge of the transition state is stabilized by this metal ion.

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Answer 4

Textbook Question

Chapter 24, Problem 1P

How does the metal ion in carboxypeptidase A increase the enzyme’s catalytic activity

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The metal ion in carboxypetidase A increases the catalytic activity of the enzyme; this has to be explained.

Explanation of Solution

Carboxypetidase A is a digestive enzyme. It hydrolyzes the carboxyl terminal peptide bond and it prefers bulky and aliphatic residues.

Proposed mechanism for the catalytic reaction of carboxypetidase is given in figure 1.

Organic Chemistry, Chapter 24, Problem 1P

Figure 1

The metal ion present in the carboxypeptidase A enzyme is Zinc metal.

This metal ion (Zn2+) makes the carbonyl (C=O) carbon more susceptible to nucleophilic addition, increases the nucleophilicity of water molecule by making it like a hydroxide ion, and the negative charge of the transition state is stabilized by this metal ion.

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Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The metal ion in carboxypetidase A increases the catalytic activity of the enzyme; this has to be explained.

Explanation of Solution

Carboxypetidase A is a digestive enzyme. It hydrolyzes the carboxyl terminal peptide bond and it prefers bulky and aliphatic residues.

Proposed mechanism for the catalytic reaction of carboxypetidase is given in figure 1.

Organic Chemistry, Chapter 24, Problem 1P

Figure 1

The metal ion present in the carboxypeptidase A enzyme is Zinc metal.

This metal ion (Zn2+) makes the carbonyl (C=O) carbon more susceptible to nucleophilic addition, increases the nucleophilicity of water molecule by making it like a hydroxide ion, and the negative charge of the transition state is stabilized by this metal ion.

Answer 8

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The metal ion in carboxypetidase A increases the catalytic activity of the enzyme; this has to be explained.

Explanation of Solution

Carboxypetidase A is a digestive enzyme. It hydrolyzes the carboxyl terminal peptide bond and it prefers bulky and aliphatic residues.

Proposed mechanism for the catalytic reaction of carboxypetidase is given in figure 1.

Organic Chemistry, Chapter 24, Problem 1P

Figure 1

The metal ion present in the carboxypeptidase A enzyme is Zinc metal.

This metal ion (Zn2+) makes the carbonyl (C=O) carbon more susceptible to nucleophilic addition, increases the nucleophilicity of water molecule by making it like a hydroxide ion, and the negative charge of the transition state is stabilized by this metal ion.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Interpretation Introduction

Interpretation:

The metal ion in carboxypetidase A increases the catalytic activity of the enzyme; this has to be explained.

Answer 12

Explanation of Solution

Carboxypetidase A is a digestive enzyme. It hydrolyzes the carboxyl terminal peptide bond and it prefers bulky and aliphatic residues.

Proposed mechanism for the catalytic reaction of carboxypetidase is given in figure 1.

Organic Chemistry, Chapter 24, Problem 1P

Figure 1

The metal ion present in the carboxypeptidase A enzyme is Zinc metal.

This metal ion (Zn2+) makes the carbonyl (C=O) carbon more susceptible to nucleophilic addition, increases the nucleophilicity of water molecule by making it like a hydroxide ion, and the negative charge of the transition state is stabilized by this metal ion.

Answer 13

Ch. 24.1 - Prob. 2P Ch. 24.1 - Prob. 3P Ch. 24.2 - How many conjugated double bonds are there in a....Ch. 24.2 - Instead of adding to the 4a position and...Ch. 24.2 - Prob. 7P Ch. 24.3 - Prob. 8P Ch. 24.3 - Acetolactate synthase is another TPP-requiring...Ch. 24.3 - Acetolactate synthase transfers the acyl group of...Ch. 24.5 - Prob. 12P Ch. 24.5 - The enzyme that catalyzes the C C bond cleavage...

How does the metal ion in carboxypeptidase A increase the enzyme’s catalytic activity

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