Chapter 23.4, Problem 5TY

Interpretation Introduction

Interpretation:

The difference in retention time required for an adequate resolution of 1.5 has to be calculated.

Concept introduction:

Resolution:

The resolution of two peaks in chromatography is given by

$\begin{array}{l}\text{Resolution}\text{=}\frac{{\text{Δt}}_{\text{r}}}{{\text{w}}_{\text{av}}}\text{=}\frac{{\text{ΔV}}_{\text{r}}}{{\text{w}}_{\text{av}}}\text{=}\frac{\text{0}{\text{.589Δt}}_{\text{r}}}{{\text{w}}_{\text{1/2av}}}\\ \text{Where,}\\ {\text{Δt}}_{\text{r}}\text{-}\text{the}\text{separation}\text{time}\text{between}\text{peaks}\\ \text{ΔV}\text{-}\text{the}\text{separation}\text{volume}\text{between}\text{peaks}\\ {\text{w}}_{\text{av}}\text{-}\text{average}\text{width}\text{of}\text{two}\text{peaks}\text{in}\text{corresponding}\text{units}\end{array}$