Concept explainers
(a)
Interpretation:
The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined.
Concept introduction:
Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.
(b)
Interpretation:
The
Concept introduction:
Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.
(c)
Interpretation:
The evidences that suggest that ATP hydrolysis is required for proteolysis, not just the presence of ATP, should be determined.
Concept introduction:
Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.
(d)
Interpretation:
The reason for differences in peptide digestion and protein digestion should be determined.
Concept introduction:
Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.
(e)
Interpretation:
The reason for the differences between protein digestion and peptide digestion should be determined.
Concept introduction:
Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.
(f)
Interpretation:
If Thermoplasma PAN can augment protein digestion by proteasomes from other organisms should be determined.
Concept introduction:
Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.
(g)
Interpretation:
The significance of the stimulation of the stimulation of rabbit muscle proteasome by Thermoplasma PAN should be determined.
Concept introduction:
Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.
Want to see the full answer?
Check out a sample textbook solutionChapter 23 Solutions
Biochemistry
- a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)arrow_forwarda) What are the differences between the Direct & Indirect Immunofluorescence Assays? b) What are the advantages of the Indirect Immunofluorescence Assays? c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?arrow_forwardCalculate the number of ATP produced from oxidation of 1 molecule of glucosearrow_forward
- Example 1: 1. Suppose an enzyme (MW = 5,000 g/mole) has a concentration of 0.05 mg/L. If the kcat is 1 x 10 s, what is the theoretical maximum reaction velocity for the enzyme? A) 1050 µM/s. B) 100 µM/s. C) 150 μM/s. D) 105 μM/s.arrow_forwardIn 1956, E. P. Kennedy and S. B. Weiss published their study of membrane lipid phosphatidylcholine (lecithin) synthesis in rat liver. Their hypothesis was that phosphocholine joined with some cellular component to yield lecithin. In an earlier experiment, incubating 32 P-labeled phosphocholine at physiological temperature (37 °C) with broken cells from rat liver yielded labeled lecithin. This became their assay for the enzymes involved in lecithin synthesis. Determine the optimal pH for this enzyme and characterize the enzyme activity at different pH values. -O-P-O-CH2-CH₁₂-N(CH3)3 Phosphocholine H₂C-O-C-R HC-O-C-R2 + + + Cell fraction + ? HC-O-P-O-CH₁₂-CH₂-N(CH), O Phosphatidylcholine The researchers then centrifuged the broken cell preparation to separate the membranes from the soluble proteins. They tested three preparations: whole extract, membranes, and soluble proteins. Table 1 summarizes the results. Table 1: Cell fraction requirement for incorporation of 32p-phosphocholine into…arrow_forwardResearchers isolated an unknown substance, X, from rabbit muscle. They determined its structure from the following observations and experiments. (a) Qualitative analysis showed that X was composed entirely of C, H, and O. A weighed sample of X was completely oxidized and the H2O and CO2 produced were measured. This quantitative analysis revealed that X contained 40.00% C, 6.71% H, and 53.29% O by weight. (b) The molecular mass of X, as determined by mass spectrometry, was 90.00 atomic mass units (u). (c) Infrared spectroscopy showed that X contained one double bond. (d) X dissolved readily in water, and the solution demonstrated optical activity when tested in a polarimeter. (e) The aqueous solution of X is acidic. What is the empirical formula of X?arrow_forward
- Show work. don't give Ai generated solution....give correct solutionarrow_forwardBiochemistry What is the process of "transamination" in either the muscles or the liver, that involves keto acid or glutamic acid? Please explain how the steps work. Thank you!arrow_forwardBiochemistry Please help. Thank you What is the importance of glutamic acid in the metabolism of nitrogen from amino acids? (we know therole; it’s used to remove the nitrogen from amino acids so that the remaining carbon skeleton can bebroken down by the “usual” pathways, but what is the important, unique role that only glutamicacid/glutamate can do?)arrow_forward
- Biochemistry Please help. Thank you When carbamyl phosphate is joined to L-ornathine, where does the energy for the reaction come from?arrow_forwardBiochemistry Question Please help. Thank you What is the function of glutamate dehydrogenase?arrow_forwardBiochemistry Question Please help. Thank you How and why does a high protein diet affect the enzymes of the urea cycle?arrow_forward
- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage LearningBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781938168130Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark WomblePublisher:OpenStax College
- Biology (MindTap Course List)BiologyISBN:9781337392938Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. BergPublisher:Cengage LearningBiology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage LearningConcepts of BiologyBiologyISBN:9781938168116Author:Samantha Fowler, Rebecca Roush, James WisePublisher:OpenStax College