(a) Interpretation: The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined. Concept introduction: Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

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Answer 1

Question

Chapter 23, Problem 42P

Interpretation Introduction

(a)

Interpretation:

The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(b)

Interpretation:

The nucleotide required for protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(c)

Interpretation:

The evidences that suggest that ATP hydrolysis is required for proteolysis, not just the presence of ATP, should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(d)

Interpretation:

The reason for differences in peptide digestion and protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(e)

Interpretation:

The reason for the differences between protein digestion and peptide digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(f)

Interpretation:

If Thermoplasma PAN can augment protein digestion by proteasomes from other organisms should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(g)

Interpretation:

The significance of the stimulation of the stimulation of rabbit muscle proteasome by Thermoplasma PAN should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD. The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?

Answer 2

Question

Chapter 23, Problem 42P

Interpretation Introduction

(a)

Interpretation:

The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(b)

Interpretation:

The nucleotide required for protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(c)

Interpretation:

The evidences that suggest that ATP hydrolysis is required for proteolysis, not just the presence of ATP, should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(d)

Interpretation:

The reason for differences in peptide digestion and protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(e)

Interpretation:

The reason for the differences between protein digestion and peptide digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(f)

Interpretation:

If Thermoplasma PAN can augment protein digestion by proteasomes from other organisms should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(g)

Interpretation:

The significance of the stimulation of the stimulation of rabbit muscle proteasome by Thermoplasma PAN should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

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Answer 3

Question

Chapter 23, Problem 42P

Interpretation Introduction

(a)

Interpretation:

The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(b)

Interpretation:

The nucleotide required for protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(c)

Interpretation:

The evidences that suggest that ATP hydrolysis is required for proteolysis, not just the presence of ATP, should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(d)

Interpretation:

The reason for differences in peptide digestion and protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(e)

Interpretation:

The reason for the differences between protein digestion and peptide digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(f)

Interpretation:

If Thermoplasma PAN can augment protein digestion by proteasomes from other organisms should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(g)

Interpretation:

The significance of the stimulation of the stimulation of rabbit muscle proteasome by Thermoplasma PAN should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

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Answer 4

Question

Chapter 23, Problem 42P

Interpretation Introduction

(a)

Interpretation:

The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(b)

Interpretation:

The nucleotide required for protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(c)

Interpretation:

The evidences that suggest that ATP hydrolysis is required for proteolysis, not just the presence of ATP, should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(d)

Interpretation:

The reason for differences in peptide digestion and protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(e)

Interpretation:

The reason for the differences between protein digestion and peptide digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(f)

Interpretation:

If Thermoplasma PAN can augment protein digestion by proteasomes from other organisms should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(g)

Interpretation:

The significance of the stimulation of the stimulation of rabbit muscle proteasome by Thermoplasma PAN should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

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Answer 5

Chapter 23, Problem 42P

Interpretation Introduction

(a)

Interpretation:

The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(b)

Interpretation:

The nucleotide required for protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(c)

Interpretation:

The evidences that suggest that ATP hydrolysis is required for proteolysis, not just the presence of ATP, should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(d)

Interpretation:

The reason for differences in peptide digestion and protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(e)

Interpretation:

The reason for the differences between protein digestion and peptide digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(f)

Interpretation:

If Thermoplasma PAN can augment protein digestion by proteasomes from other organisms should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Interpretation Introduction

(g)

Interpretation:

The significance of the stimulation of the stimulation of rabbit muscle proteasome by Thermoplasma PAN should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 6

Chapter 23, Problem 42P

Interpretation Introduction

(a)

Interpretation:

The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 7

Chapter 23, Problem 42P

Interpretation Introduction

(a)

Interpretation:

The effect of PAN on archaeal proteasome activity in the absence of nucleotides should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzymes that help in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The nucleotide required for protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The nucleotide required for protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The evidences that suggest that ATP hydrolysis is required for proteolysis, not just the presence of ATP, should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The evidences that suggest that ATP hydrolysis is required for proteolysis, not just the presence of ATP, should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 12

Interpretation Introduction

(d)

Interpretation:

The reason for differences in peptide digestion and protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 13

Interpretation Introduction

(d)

Interpretation:

The reason for differences in peptide digestion and protein digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 14

Interpretation Introduction

(e)

Interpretation:

The reason for the differences between protein digestion and peptide digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 15

Interpretation Introduction

(e)

Interpretation:

The reason for the differences between protein digestion and peptide digestion should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 16

Interpretation Introduction

(f)

Interpretation:

If Thermoplasma PAN can augment protein digestion by proteasomes from other organisms should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 17

Interpretation Introduction

(f)

Interpretation:

If Thermoplasma PAN can augment protein digestion by proteasomes from other organisms should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 18

Interpretation Introduction

(g)

Interpretation:

The significance of the stimulation of the stimulation of rabbit muscle proteasome by Thermoplasma PAN should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 19

Interpretation Introduction

(g)

Interpretation:

The significance of the stimulation of the stimulation of rabbit muscle proteasome by Thermoplasma PAN should be determined.

Concept introduction:

Proteasomes are the protein complexes; whose function is to degrade the damaged proteins by the process of proteolysis. Proteolysis process breaks the peptide bonds between the amino acids. Further, the enzyme that helps in this process is termed as proteases. In eukaryotes, proteasomes are found in the nucleus and cytoplasm of the cell.

Answer 20

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Answer 21

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Answer 22

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Answer 23

Ch. 23 - Prob. 1P Ch. 23 - Prob. 2P Ch. 23 - Prob. 3P Ch. 23 - Prob. 4P Ch. 23 - Prob. 5P Ch. 23 - Prob. 6P Ch. 23 - Prob. 7P Ch. 23 - Prob. 8P Ch. 23 - Prob. 9P Ch. 23 - Prob. 10P

Solution Summary: The author analyzes the effect of PAN on archaeal proteasome activity in the absence of nucleotides.

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