The name of amino acids that needs an enzyme complex similar to the pyruvate dehydrogenase complex and alpha-ketoglutarate dehydrogenase complex should be given. Also, the name of that enzyme complex should be given. Concept introduction: Amino acids can be defined as the organic compounds which combine to form proteins. Proteins act as the building blocks of life. When proteins are broken down then amino acids are left behind. Amino acids are of two types: glucogenic amino acid and ketogenic amino acid. Pyruvate dehydrogenase enzyme complex is required for the linking of glycolysis and TCA.

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Chapter 23, Problem 31P

Interpretation Introduction

Interpretation:

The name of amino acids that needs an enzyme complex similar to the pyruvate dehydrogenase complex and alpha-ketoglutarate dehydrogenase complex should be given. Also, the name of that enzyme complex should be given.

Concept introduction:

Amino acids can be defined as the organic compounds which combine to form proteins. Proteins act as the building blocks of life. When proteins are broken down then amino acids are left behind. Amino acids are of two types: glucogenic amino acid and ketogenic amino acid. Pyruvate dehydrogenase enzyme complex is required for the linking of glycolysis and TCA.

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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD. The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?