Biology: Life on Earth with Physiology (11th Edition)
11th Edition
ISBN: 9780133923001
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Textbook Question
Chapter 22.1, Problem 1CYL
- describe the features that distinguish plants from other kinds of organisms?
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a) The lux operon is under positive control. Based on this information, does the luxR regulator sequence make a repressor protein or an activator protein?
b) How will binding of this complex affect RNA polymerase? Remember this operon is under positive control.
c) AHL is a signal molecule that V. fisheri makes to communicate with neighboring bacterial cells. This molecule can diffuse outside of the cell and into another bacterial cell in close proximity. This type of communication between bacterial cells is known as quorum sensing. If bacterial cell density is low how will this affect the lux operon? What will happen if the density is high?
1) Given an mRNA with the following sequence, please translate the codons to a chain of amino acids. Use the codon chart below.5’AUG/CCU/GCU/UAC/CGG/GAG/UAA3’
“met-_________-_________-_________-_________-_________”STOP”
2) Assuming the original polypeptide chain below, match each type of point mutation with the polypeptide chain that results.
Original polypeptide: Pro-Thr-Ser-Leu-Leu-His-Asn
A. Missense
B. Silent
C. Nonsense
D. Frameshift
______ Pro-Thr-Ser-STOP
______ Pro-Thr-Ser-Leu-Ile-His-Asn
______ Pro-Thr-Ser-Leu-Leu-His-Asn
_______ Pro-Thr-His-Cys-Tyr-Thr
Chapter 22 Solutions
Biology: Life on Earth with Physiology (11th Edition)
Ch. 22.1 - describe the features that distinguish plants from...Ch. 22.2 - describe the probable ancestor of plants?Ch. 22.2 - identify the closest living relatives of plants...Ch. 22.2 - describe the adaptations that equip plants for...Ch. 22.2 - In stark contrast to the stinking corpse lily and...Ch. 22.3 - Why are all nonvascular plants short?Ch. 22.3 - In each of these photos, is the pictured structure...Ch. 22.3 - Can you think of some adaptations that help...Ch. 22.3 - What are the advantages and disadvantages of wind...Ch. 22.3 - Which Plant Provide Us with the Most Food?
Ch. 22.3 - Prob. 1CYLCh. 22.3 - describe the major plant taxonomic groups and...Ch. 22.3 - describe the key steps in the life cycles of...Ch. 22.3 - The stinking corpse lily harms species it...Ch. 22.4 - In the initial trials with Argemone mexicana in...Ch. 22.4 - describe some of the effects that plants have on...Ch. 22.4 - Perhaps surprisingly, a parasitic lifestyle is not...Ch. 22 - In an alternation of generations life cycle,...Ch. 22 - Which of the following are not nonvascular plants?...Ch. 22 - Which of the following structures is present in...Ch. 22 - In which of the following is the gametophyte stage...Ch. 22 - Prob. 5MCCh. 22 - Scientists hypothesize that the ancestors of...Ch. 22 - Plant adaptations to life on land include a(n)...Ch. 22 - Seedless vascular plants must reproduce when...Ch. 22 - Three groups of nonvascular plants are __________,...Ch. 22 - What is meant by alternation of generations? What...Ch. 22 - Prob. 2RQCh. 22 - Describe evolutionary trends in the life cycles of...Ch. 22 - From which algal group did green plants probably...Ch. 22 - Prob. 5RQCh. 22 - Prob. 6RQCh. 22 - Prob. 7RQCh. 22 - Prob. 8RQCh. 22 - Prob. 9RQCh. 22 - Prob. 1ACCh. 22 - Prob. 2AC
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- a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates? b) Predict the growth you would expect to see on each of the following plates: – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________arrow_forward1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forward
- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forward
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