Nester's Microbiology: A Human Perspective
9th Edition
ISBN: 9781259709999
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Chapter 22, Problem 9MC
Summary Introduction
Introduction:
Thecells are grown in the controlled conditions or artificial environment once they are taken from a source such as an animal or plant. This process is called culturing of a cell or cell culture. On the contrary, to this, a cell-free media or a system is a tool used for in vitro studies in which complex interactions within the entire cell is avoided to study biological reactions which occur within the cells.
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 22 Solutions
Nester's Microbiology: A Human Perspective
Ch. 22 - Prob. 1SACh. 22 - Prob. 2SACh. 22 - How is impetigo spread?Ch. 22 - Prob. 4SACh. 22 - Prob. 5SACh. 22 - Describe the progression of the rash of varicella.Ch. 22 - Prob. 7SACh. 22 - Prob. 8SACh. 22 - What is the significance of rubella viremia during...Ch. 22 - How does a person contract warts?
Ch. 22 - Prob. 1MCCh. 22 - Staphylococcus aureus can be responsible for all...Ch. 22 - Prob. 3MCCh. 22 - Prob. 4MCCh. 22 - Which of the following statements is true of...Ch. 22 - Prob. 6MCCh. 22 - Prob. 7MCCh. 22 - Prob. 8MCCh. 22 - Prob. 9MCCh. 22 - Prob. 10MCCh. 22 - Prob. 1ACh. 22 - Prob. 2ACh. 22 - Prob. 1CTCh. 22 - Why might it be more difficult to eliminate a...
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