Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 22, Problem 3CT
Indicators are used in autoclaving to ensure that sterilization is complete. One type of chemical indicator turns color when it has reached a specific temperature; the other type turns color when it has reached a specified temperature and been exposed to steam. Which type of indicator should be used?
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Which physical or chemical agent would be the best choice for sterilizing the following items: glass pipettes, tryptic soy broth tubes, nutrient agar, antibiotic solution, interior of a biological safety cabinet, wrapped package of plastic Petri plates? Explain your choices.
In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either.
Materials:
200-ml Erlenmeyer flask Stove
500-ml Erlenmeyer flask Autoclave
10-mL graduated cylinder Analytical balance
100-ml graduated cylinder pH meter
Spatula Stirring rod
100-mL beaker Test tubes
Distilled water Petri dish
Stirring rod Alcohol lamp
Glass dropper
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Chapter 22 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 22 - Prob. 1QCh. 22 - Prob. 2QCh. 22 - Give an example of a nonlaboratory use of each of...Ch. 22 - Define the term pasteurization. What is the...Ch. 22 - Explain why fungi and Bacillus sometimes grow...Ch. 22 - Assume that a DRT value for autoclaving a culture...Ch. 22 - Indicators are used in autoclaving to ensure that...Ch. 22 - A biological indicator used in autoclaving is a...Ch. 22 - The source of Legionella causing...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- During media preparation, you observed that the agar medium did not solidify after sterilization even after incubating it for 1 hour at room temperature. What could be the reason behind this?arrow_forwardIn the laboratory, autoclaving is required to prepare media for the growth of microorganism. What type of method of control does this involve? Dry Heat Moist Heat Chemical Radiation All of the optionsarrow_forwardIn pharmaceutical companies, Sterilization is an important step for quality assurance and approval of pharmaceutical products. Based on this concept, answer the following questions. Heat sensitive liquids need special methods for sterilization. Explain the methods applied in details.arrow_forward
- Give two reasons for heating the slide after the smear is air dried?arrow_forwardOther than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.arrow_forwardIn sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down. For "autoclaving" For Dry heat oven Can be sterilized only sterilization with either Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-ml graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-ml beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperarrow_forward
- Make a list of the different types of sterilization, including the method, the principle, the materials that can be sterilized, the required temperature and time, and the required temperature and time. Method Principle Apparatus Temp/Time Materialsarrow_forwardOven sterilization at 121˚C was performed on glassware for 15 minutes only. Effect: Rationale:arrow_forwardWhat is the purpose of flaming an inoculating loop? How will you know when you have flamed the loop or needle long enough? Why is it necessary to cool the inoculating loop prior to obtaining the bacterial sample? In which direction should you move the inoculating loop in the Bunsen burner flame? (i.e. from the handle to the loop or from the loop to the handle)arrow_forward
- Given the following common laboratory materials, which of the three methods of heat sterilization do you think is the most appropriate and practical to use in sterilizing them ? Why do you think so ? a) empty flask b)L-rod (glass rod c)10 ml glass pipet d) wire needles e)antibiotic solutionarrow_forwardFor sterilization to occur correctly it must include; heat and pressure: true Falsearrow_forwardThe following data were obtained from pour plates used to test the effectiveness of a food preservative. Two samples of cottage cheese were inoculated with bacteria; the preservative was added to one sample. After incubation, samples of the cottage cheese (control) and samples treated with the preservative (experimental) were diluted and plated on nutrient agar. Calculate the number of bacteria. Was the preservative effective? Dilution Amount Plated (mL) Number of Colonies CFU/mL Control 1:400 1 160 Experimental 1:200 0.1 32arrow_forward
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