Exploring Chemical Analysis
Exploring Chemical Analysis
5th Edition
ISBN: 9781429275033
Author: Daniel C. Harris
Publisher: Macmillan Higher Education
Question
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Chapter 22, Problem 22.15P

(a)

Interpretation Introduction

Interpretation:

Whether higher or lower percentage of acetonitrile should be used in the next run has to be explained.

Concept Introduction:

In reversed phase chromatography, stationary phase is less polar than the mobile phase. So, the stationary phase will retain the non-polar component more tightly and polar component will be eluted first. Hence to increase the resolution of the peaks, the polarity of mobile phase should be increased.

(b)

Interpretation Introduction

Interpretation:

Whether higher or lower percentage of hexane should be used in the next run has to be explained.

Concept Introduction:

In normal phase chromatography, stationary phase is more polar than the mobile phase. So, the stationary phase will retain the polar component more tightly and non-polar component will be eluted first. Hence to increase the resolution of the peaks, the polarity of mobile phase should be decreased.

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