Chapter 21, Problem 52P

Interpretation Introduction

Interpretation:

The amino acid having greatest amount of negative charge at pH 7.4 has to be identified from amino acids leucine and asparagine.

Concept introduction:

The isoelectric point $\text{(pI)}$ is the $\text{pH}$ at which the amount of positive charge and negative charge on an amino acid becomes balanced.

The value of $pI$ is depends upon the $p{K}_a$ value of protonated amino group and $p{K}_a$ value of carboxyl group of an amino acid.