Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Chapter 21, Problem 4TYK
Summary Introduction
Introduction: Proteomics and genomics enable molecular biologists to approach the study of life. The technological advances facilitated the study of systems biology.
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Why can the transcriptome not be used to predict the proteome with complete accuracy?
a. It cannot be sequenced like the genome can be.
b. The transcriptome is too dynamic to be used to make predictions.
c. Not all genes are transcribed.
d. Many transcripts are alternatively spliced to produce different proteins.
Proteomics is used to discovera. what genes are active in what cells.b. what proteins are active in what cells.c. the structure and function of proteins.d. how proteins interact.e. All except a are correct.
What is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA seq (RNA seq)? d. What is the main difference between a genomic and a transcriptome study?
Chapter 21 Solutions
Study Guide for Campbell Biology
Ch. 21 - In what ways would third-generation sequencing be...Ch. 21 - Prob. 2IQCh. 21 - Refer to the organisms listed in Table 21.1 in...Ch. 21 - Explain why retrotransposons always move by the...Ch. 21 - For each of the following types of DNA sequences...Ch. 21 - Prob. 6IQCh. 21 - Prob. 7IQCh. 21 - If all Hox genes contain the same or very similar...Ch. 21 - About 25% of the human genome relates to the...Ch. 21 - Prob. 2SYK
Ch. 21 - Which of the following has decreased the time and...Ch. 21 - Prob. 2TYKCh. 21 - In the process called gene annotation, computer...Ch. 21 - Prob. 4TYKCh. 21 - Prob. 5TYKCh. 21 - Prob. 6TYKCh. 21 - What is a pseudogene? a. a gene that has been...Ch. 21 - Prob. 8TYKCh. 21 - Which of the following is common to both...Ch. 21 - Prob. 10TYKCh. 21 - Prob. 11TYKCh. 21 - Prob. 12TYKCh. 21 - Prob. 13TYKCh. 21 - Prob. 14TYKCh. 21 - Compared to genes in mice and chimpanzees, most...
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- 1a) Why is it possible for you to study the eye colour gene by extracting cheek cells? a. Because the nucleus of every cell in the human body contains the same genetic information. b. Because the cheek cells are located near the cells of the eye and so they are able to exchange DNA. c. Because all genes in the human body are expressed at all times so it is easy to study them. d. All of the above are possible explanations. 1b) What is the purpose of heating the sample to 75°C following addition of the 0.2M NaOH solution? a. To denature the histone proteins that are keeping the DNA tightly coiled. b. To ensure that all the DNA is removed from the swab in preparation for PCR. c. To breakdown the cheek cell membrane to release the DNA from the cell. d. It breaks down the circular DNA down into linear fragments so that they will be easier to visualize.iarrow_forwardSequencing a genome and identifying individual genes are processes typically carried out - A. via manual transcription by a team of genetic scientists. B. by computers using high-throughput methods. C. by hand with a team of information scientists. D. using experimental processes.arrow_forwardGive typing answer with explanation and conclusion If you want to identify genes linked to autism in a mouse model, which genetic approach or approaches could you use? (Mark all that apply) A) Reverse Genetics B) Forward Genetics C) Optogenetics D) Population Geneticsarrow_forward
- Identify the word or phrase that is the best match (some will remain blank). (Mouse Genome Informatics, GeneChip array, Confounding variable, 45,000, BLAST, 600, Aceview, BioGPS, SMART, minimize, 17, UCSC Genome Browser, External validity) A. Median number of copies of an mRNA in a cell B. Patterns and sizes of introns and exons C. H3K4me3 locations on chromosomes D. Each treatment given to a different litter of mice E. Find list of knockout mice for a gene D. Find similar proteins F. Random variation G. Tissue expression pattern of a gene H. The extent to which results can be extrapolatedarrow_forwardSite-directed mutagenesis can be used to: a. treat diseases by gene therapy. b. make single residue mutation of a protein. c. make human insulin for treatment of diabetes. d. determine protein structures. e. determine DNA sequencingarrow_forwardThe goal of most gene therapies is to insert a healthy copy of a gene into the genome. Besides CRISPR, techniques for integrating DNA into the genome do not target that DNA to a particular genomic location and thus different cells end up with the DNA sequence in different positions. A. It has been shown that where the healthy gene integrates is heavily influenced by chromatin. Why? B. The same gene integrated into different locations in the genome will be expressed differently. Why? C. Over time the expression of an integrated gene will change, usually decreasing. Describe how you think the cell downregulates expression of an integrated gene.arrow_forward
- What data have been complied in the Human Protein Atlas? https://www.proteinatlas.org/about A.The Human Protein Atlas was created by integrating different omics technologies such as antibody-based imaging, proteomic analysis by mass spectrometry, transcriptomics and systems biology. B.The Human Protein Atlas was established by examining the distribution of proteins in a diseased tissue and inferring where the protein localize in a healthy tissue. C.The Human Protein Atlas was created by creating a list of all know proteins in alphabetical order. D.The Human Protein Atlas was created immunoblot analysis of proteins in different tissues.arrow_forwardBriefly answer the question: Exome sequencing to identify a mutation that could cause a particular set of symptoms in a patient can reveal another genetic condition that has not yet been detected. Under what circumstances, if any, do you think patients should receive such "secondary findings"?arrow_forwardComparative proteomics involves comparing the proteomes from two different cells or tissues from different conditions. In some cancer cells the Retinoblastoma protein often appears to have a slightly higher molecular mass than in noncancerous cells even though the proteins have identical amino acid sequences. What might explain this difference? a. Posttranslational modification b. Alternative splicing c. Association with different proteins d. Mutation of the gene encoding the proteinarrow_forward
- Give detailed Solution with explanation neededarrow_forwardGenome annotation refers to ... 1.) lining up overlapping regions in short shotgun sequencing reads to assemble larger contiguous DNA sequences (contigs/scaffolds). 2.) using long-read sequencing platforms (such as PacBio or Oxford Nanopore) to gather information about the epigenetic status of each region of a sequenced genome. 3.) the process of predicting which parts of a genome sequence code for functional products (such as protein-coding genes), what those products do, and assigning them names. 4.) sequencing messengeRNA measure the relative expression levels of genes in one or more tissue samples.arrow_forwardCheck all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing vs. whole genome illumina (NGS) sequencing. A. You don't need to trim the reads for 16S sequencing, but you do for whole genome sequencing. B. Whole genome sequencing files will have more reads in them because genomes are bigger than 16S amplicons. C. The read alignment and contig assembly steps would probably be harder for whole genome sequencing. D. The 16S molecules being sequenced are always DNA, while for whole genome sequencing they could be RNA or DNA going into the illumina sequencer. E. NGS for 16S and whole genome sequencing can both use sample indexes/barcodes to maximize efficiency. F. Whole genome sequencing doesn't necessarily require you to know any of the sequences/targets before hand to design specific primers for.arrow_forward
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