To explain: The role of complementary base pairing during RT-PCR.
Concept introduction: The gene functions can be studied through its expression in a particular cell and its interaction with other genes. To study the gene expression, specific mRNAs corresponding to the gene of interest are to be identified. The methods used for these include RT-PCR, DNA microarray analysis, and RNA sequencing. These are based on the base-pairing between the nucleotides.
To explain: The role of complementary base pairing during microarray analysis.
Concept introduction: DNA microarray analysis is a technique used in molecular biology for identifying the expression levels of genes where collection of microscopic DNA spots is attached to a solid surface known as DNA chip.
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Campbell Biology (10th Edition)
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