Campbell Biology (10th Edition)
10th Edition
ISBN: 9780321775658
Author: Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson
Publisher: PEARSON
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Textbook Question
Chapter 20, Problem 4TYU
A paleontologist has recovered a bit of tissue from the 400-year old preserved skin of an extinct dodo (a bird). Tocompare a specific region of the DNA from a sample vvith DNA from living birds, which of the following would be most useful for increasing the amount of dodo DNA available for testing?
- (A) SNPanalysis
- (B) Polymerase chain reaction (PCR)
- (C) electroporation
- (D) gel electrophoresis
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Given the situation: A paleontologist has recovered a tiny bit of organic material fromthe 400-year-old preserved skin of an extinct dodo. She would like to compare DNA from the sample with DNA from living birds.Which of the following would be most useful for increasing theamount of DNA available for testing?
Option a.) restriction fragment analysis
Option b.) polymerase chain reaction
Option c.) electrophoresis
Which of the following statements concerning recombinantDNA technology is false?(a) Thus far, no illnesses in laboratory workers have beentraced to genetic recombinants.(b) Production of large amounts of proteins such as insulinand human growth hormone has been made possible us-ing recombinant DNA technology.(c) Recombinant DNA technology offers specific benefitsto the scientific, medical, and general populations.(d) Mutant strains of bacteria produced by genetic re-combination are often unable to survive in the naturalenvironment.(e) Recombinant DNA technology provides a high degreeof risk to the health of the general populations.
In DNA technology, the term vector can refer to(A) the enzyme that cuts DNA into restrictionfragments.(B) the sticky end of a DNA fragment.(C) a SNP marker.(D) a plasmid used to transfer DNA into a living cell.
Chapter 20 Solutions
Campbell Biology (10th Edition)
Ch. 20.1 - Prob. 1CCCh. 20.1 - Prob. 2CCCh. 20.1 - What are some potential difficulties in using...Ch. 20.1 - Prob. 4CCCh. 20.2 - Prob. 1CCCh. 20.2 - Prob. 2CCCh. 20.3 - Based on current knowledge, how would you explain...Ch. 20.3 - Prob. 2CCCh. 20.3 - Prob. 3CCCh. 20.4 - What is the advantage of using stem cells for gene...
Ch. 20.4 - Prob. 2CCCh. 20.4 - Prob. 3CCCh. 20 - Describe how the process of gene doning results in...Ch. 20 - What useful Information is obtained by detecting...Ch. 20 - Describe how, using mice. a researcher could carry...Ch. 20 - What factors affecf whether a given genetic...Ch. 20 - In DNA technology, the term vector can refer to...Ch. 20 - Which of the following tools of DNA technology is...Ch. 20 - Prob. 3TYUCh. 20 - A paleontologist has recovered a bit of tissue...Ch. 20 - Prob. 5TYUCh. 20 - Which of the following is not true of cDNA...Ch. 20 - Expression of a cloned eukaryotic gene in a...Ch. 20 - Which Ii of the following sequences in...Ch. 20 - Prob. 9TYUCh. 20 - Prob. 10TYUCh. 20 - EVOLUTlON CONNECTION Ethical considerations aside,...Ch. 20 - Prob. 12TYUCh. 20 - Prob. 13TYUCh. 20 - The water in the Yellowstone National Park hot...
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- (a) Describe the actions with reason that should be taken under the following situations: (i) An unlabelled collection tube with a requisition for a V Leiden test is received in the laboratory. (ii) After PCR, the amplification control has failed to yield a product. (iii) DNA is stored overnight in a refrigerator set at 8oC but reads 14 oC. (iv) An isolated DNA sample is to be stored for at least 6 months. (v) The expiration date on a reagent has passed. (b) Explain briefly about genetic testing for new-born, including the importance of genetic testing and the disorder that genetic testing can detect.arrow_forwardQuantitative PCR differs from regular PCR in that it uses [A] to [B] the amount of [C] in a sample. It cannot quantify [D] unless it is first made into [F]. Match each of the following to its appropriate letter: quantify, cDNA, RNA, DNA or RNA, fluorescence. 1) A 2) B 3) C 4) D 5) E Here are the choices for the questions a) quantify b) RNA c) cDNA d) fluorescence e) DNA or RNAarrow_forward(A) After three cycles of PCR, how many DNA molecules are present that correspond precisely to the desired amplification product? (B) What about after 5 cycles. Assume that we started with one molecule in each case, and that the reaction is perfectly efficient.arrow_forward
- (iii) What are the three (3) main cycles in PCR? (iv) Discuss the processes at each PCR cycle mentioned in Q3 a) (iii).arrow_forwardmolecules continues to double every temperature cycle (referred to as a PCR cycle). (a) Suppose a sample containg x molecules is collected from a crime scene and is amplified by PCR. Express the number of DNA molecules as a function of the number n of PCR cycles. (b) There is a detection threshold of T molecules below which no DNA can be seen. Derive an equation for the number of PCR cycles it will take for the DNA sample Amplifying DNA Polymerase Chain Reaction (PCR) is a biochemical technique that allows scientists to take tiny samples of DNA and amplify them into large samples that can then be examined to determine the DNA sequence. (This is useful, for example, in forensic science.) The pro- cess works by mixing the sample with appropriate enzymes and then heating it until the DNA double helix separates into two individual strands. The enzymes then copy each strand, and once the sample is cooled the number of DNA molecules will have doubled. By repeatedly performing this heating…arrow_forwardThe PCR technique uses (a) heat-resistant DNA polymerase (b) reverse transcriptase (c) DNA ligase (d) restriction enzymes (e) b and carrow_forward
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