Campbell Biology (10th Edition)
10th Edition
ISBN: 9780321775658
Author: Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Question
Chapter 20, Problem 10TYU
Summary Introduction
To draw: The cloning procedure of the given aardvark gene sequence using the bacterial plasmid.
Concept introduction:
Polymerase chain reaction (PCR) is used to amplify the gene sequence of interest tosynthesize its multiple copies. This gene fragment is then inserted into the expression vector to synthesize the protein that is encoded by the gene, which is known as cloning.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Amino
Acid Coclow
TABle
3'
Gly
Phe
Leu
(G)
(F) (L)
3-
Val
(V)
Arg (R)
Ser (S)
Ala
(A)
Lys (K)
CAG
G
Glu
Asp (E)
(D)
Ser
(S)
CCCAGUCAGUCAGUCAG
0204
C
U
A G
C
Asn
(N)
G
4
A
AGU
C
GU
(5)
AC
C
UGA
A
G5
C
CUGACUGACUGACUGAC
Thr
(T)
Met (M)
lle
£€
(1)
U
4
G
Tyr
Σε
(Y)
U
Cys (C)
C
A
G
Trp (W) 3'
U
C
A
Leu
בוט
His
Pro
(P)
££
(H)
Gin
(Q)
Arg
흐름
(R)
(L)
Start
Stop
8. Transcription and Translation Practice: (Video 10-1 and 10-2)
A. Below is the sense strand of a DNA gene. Using the sense strand, create the antisense
DNA strand and label the 5' and 3' ends.
B. Use the antisense strand that you create in part A as a template to create the mRNA
transcript of the gene and label the 5' and 3' ends.
C. Translate the mRNA you produced in part B into the polypeptide sequence making sure
to follow all the rules of translation.
5'-AGCATGACTAATAGTTGTTGAGCTGTC-3' (sense strand)
4
What is the structure and function of Eukaryotic cells, including their organelles? How are Eukaryotic cells different than Prokaryotic cells, in terms of evolution which form of the cell might have came first? How do Eukaryotic cells become malignant (cancerous)?
What are the roles of DNA and proteins inside of the cell? What are the building blocks or molecular components of the DNA and proteins? How are proteins produced within the cell? What connection is there between DNA, proteins, and the cell cycle? What is the relationship between DNA, proteins, and Cancer?
Chapter 20 Solutions
Campbell Biology (10th Edition)
Ch. 20.1 - Prob. 1CCCh. 20.1 - Prob. 2CCCh. 20.1 - What are some potential difficulties in using...Ch. 20.1 - Prob. 4CCCh. 20.2 - Prob. 1CCCh. 20.2 - Prob. 2CCCh. 20.3 - Based on current knowledge, how would you explain...Ch. 20.3 - Prob. 2CCCh. 20.3 - Prob. 3CCCh. 20.4 - What is the advantage of using stem cells for gene...
Ch. 20.4 - Prob. 2CCCh. 20.4 - Prob. 3CCCh. 20 - Describe how the process of gene doning results in...Ch. 20 - What useful Information is obtained by detecting...Ch. 20 - Describe how, using mice. a researcher could carry...Ch. 20 - What factors affecf whether a given genetic...Ch. 20 - In DNA technology, the term vector can refer to...Ch. 20 - Which of the following tools of DNA technology is...Ch. 20 - Prob. 3TYUCh. 20 - A paleontologist has recovered a bit of tissue...Ch. 20 - Prob. 5TYUCh. 20 - Which of the following is not true of cDNA...Ch. 20 - Expression of a cloned eukaryotic gene in a...Ch. 20 - Which Ii of the following sequences in...Ch. 20 - Prob. 9TYUCh. 20 - Prob. 10TYUCh. 20 - EVOLUTlON CONNECTION Ethical considerations aside,...Ch. 20 - Prob. 12TYUCh. 20 - Prob. 13TYUCh. 20 - The water in the Yellowstone National Park hot...
Knowledge Booster
Similar questions
- please fill in the empty sports, thank you!arrow_forwardIn one paragraph show how atoms and they're structure are related to the structure of dna and proteins. Talk about what atoms are. what they're made of, why chemical bonding is important to DNA?arrow_forwardWhat are the structure and properties of atoms and chemical bonds (especially how they relate to DNA and proteins).arrow_forward
- The Sentinel Cell: Nature’s Answer to Cancer?arrow_forwardMolecular Biology Question You are working to characterize a novel protein in mice. Analysis shows that high levels of the primary transcript that codes for this protein are found in tissue from the brain, muscle, liver, and pancreas. However, an antibody that recognizes the C-terminal portion of the protein indicates that the protein is present in brain, muscle, and liver, but not in the pancreas. What is the most likely explanation for this result?arrow_forwardMolecular Biology Explain/discuss how “slow stop” and “quick/fast stop” mutants wereused to identify different protein involved in DNA replication in E. coli.arrow_forward
- Molecular Biology Question A gene that codes for a protein was removed from a eukaryotic cell and inserted into a prokaryotic cell. Although the gene was successfully transcribed and translated, it produced a different protein than it produced in the eukaryotic cell. What is the most likely explanation?arrow_forwardMolecular Biology LIST three characteristics of origins of replicationarrow_forwardMolecular Biology Question Please help. Thank you For E coli DNA polymerase III, give the structure and function of the b-clamp sub-complex. Describe how the structure of this sub-complex is important for it’s function.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education