Microbiology: An Introduction
Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Chapter 20, Problem 6A

The following results were obtained from a broth dilution test for microbial susceptibility.

Antibiotic Concentration Growth Growth in Subculture
200 µg/ml  -  -
100 µg/ml  - +
50 µg/ml + +
25 µg/ml + +

a. The MIC of this antibiotic is _______.

b. The MBC of this antibiotic is _______.

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Which among the group would be affected when excessive Zn++ is present in the medium? Provide two (2) corrective actions to resolve this error.
3- Visible Growth in Tubes Growth in Petri Dishes 0 µg/ml 2 µg/ml 0 1 3 4 ÖÖÖÖÖÖÖÖ 2 5 6 7 number 1, and After addition of The figure given above summarizes an experiment carried out to test the efficacy of an antimicrobial substance. In the tube number 0, no antimicrobial substance was added into bacterial culture. 2 µg/ml of antimicrobial substance was added into tube the amount of antimicrobial was doubled for each consecutive tube. antimicrobial into tubes, tubes were incubated for overnight and after incubation tubes were observed for growth. The growth status of bacteria in each tube was shown as (+) and (-). In addition to observation of visible growth, 1 ml of sample from each tube was collected and plated into petri dishes and incubated for additional overnight. Similarly, the growth status in petri dishes was as (+) and (-). According to given information; a. Determine the minimum inhibitory concentration (MIC). b. Determine the minimum bactericidal concentration (MBC). c.…
Background: The following results were obtained from a broth dilution test for microbial susceptibility. What is the minimum bactericidal concentration of this antibiotic and explain what you based your answer on?
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