EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 20, Problem 5CT
Summary Introduction
To answer:
The diagnosis, effective treatment, animal sources of infection and the treatment to be received by the friends and caregivers of a patient suffering from seizures, disorientation and inordinate fear of water.
Introduction:
The young man has rabies. The virus infection has progressed to the brain; a vaccine will not be effective at this point.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 20 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 20 - Prob. 1TMWCh. 20 - Prob. 1EDCSCh. 20 - Prob. 1CCSCh. 20 - Prob. 2TMWCh. 20 - Prob. 2CCSCh. 20 - Prob. 2EDCSCh. 20 - Prob. 3TMWCh. 20 - Prob. 4TMWCh. 20 - Prob. 3CCSCh. 20 - Prob. 5TMW
Ch. 20 - A Protozoan Mystery A 20-year-old student was...Ch. 20 - Prob. 6TMWCh. 20 - Prob. 7TMWCh. 20 - A Very Sick Sophomore In December, a 19-year-old...Ch. 20 - Cerebrospinal fluid is _____. a. formed deep...Ch. 20 - The layer of the meninges lying closest to the...Ch. 20 - Prob. 3MCCh. 20 - Prob. 4MCCh. 20 - Prob. 5MCCh. 20 - Prob. 6MCCh. 20 - Prob. 7MCCh. 20 - Prob. 8MCCh. 20 - Prob. 9MCCh. 20 - Prob. 10MCCh. 20 - Which of the following diseases is caused most...Ch. 20 - Prob. 12MCCh. 20 - Prob. 13MCCh. 20 - Prob. 14MCCh. 20 - Prob. 15MCCh. 20 - Prob. 1FIBCh. 20 - Prob. 2FIBCh. 20 - Prob. 3FIBCh. 20 - Prob. 4FIBCh. 20 - Prob. 5FIBCh. 20 - Prob. 6FIBCh. 20 - Prob. 7FIBCh. 20 - Prob. 8FIBCh. 20 - Prob. 9FIBCh. 20 - Prob. 10FIBCh. 20 - Prob. 11FIBCh. 20 - Prob. 12FIBCh. 20 - Prob. 13FIBCh. 20 - A sexually transmitted bacterium, ________, causes...Ch. 20 - Prob. 15FIBCh. 20 - Prob. 1VICh. 20 - Indicate on the drawing where a lumbar puncture...Ch. 20 - Distinguish among the following infections:...Ch. 20 - Prob. 2SACh. 20 - Prob. 3SACh. 20 - Prob. 4SACh. 20 - Prob. 5SACh. 20 - Prob. 6SACh. 20 - Prob. 7SACh. 20 - Prob. 8SACh. 20 - Prob. 9SACh. 20 - Prob. 1CTCh. 20 - Prob. 2CTCh. 20 - Prob. 3CTCh. 20 - Prob. 4CTCh. 20 - Prob. 5CTCh. 20 - How are the actions of botulism and tetanus toxins...Ch. 20 - Prob. 7CTCh. 20 - Prob. 8CTCh. 20 - Why does the fact that M. leprae grows best at 30C...Ch. 20 - Prob. 10CTCh. 20 - Prob. 1CM
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