EP HUMAN BIOLOGY-MODIFIED MASTERING
8th Edition
ISBN: 9780134326436
Author: Johnson
Publisher: PEARSON CO
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Textbook Question
Chapter 20, Problem 3TY
Which of the following is used to separate fragments of DNA by size?
a. gel electrophoresis
b. polymerase chain reaction
c. DNA insertion into a plasmid
d. DNA splicing
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Which of the following one is liable for the conversion of covalently closed circular DNA to super coiled DNA of the plasmid?
a.Exonuclease
b.
DNA Gyras
c.
Endonuclease
d.
Topoisomerase
DEFINE THE FOLLOWING:
1) restriction enzyme
2) plasmid
3) recombinant DNA
Describe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description.
Chapter 20 Solutions
EP HUMAN BIOLOGY-MODIFIED MASTERING
Ch. 20 - Prob. 1QCCh. 20 - GM foods are created for a variety of reasons,...Ch. 20 - Should foods that contain genetically modified...Ch. 20 - Explain how DNA is sequenced.Ch. 20 -
2. Describe the enzymes used in recombinant DNA...Ch. 20 - Prob. 3CRCh. 20 - Discuss the polymerase chain reaction technique,...Ch. 20 - Prob. 5CRCh. 20 - Prob. 6CRCh. 20 - Prob. 7CR
Ch. 20 - Prob. 8CRCh. 20 - Prob. 9CRCh. 20 -
10. List the diseases that gene therapy may be...Ch. 20 - Prob. 1TYCh. 20 - Prob. 2TYCh. 20 - Which of the following is used to separate...Ch. 20 - Genetically engineered human insulin, human growth...Ch. 20 - Prob. 5TYCh. 20 - All of the following statements about transgenic...Ch. 20 -
7. "Gene pharming” refers to:
a. using transgenic...Ch. 20 - Prob. 8TYCh. 20 - Prob. 9TYCh. 20 - Prob. 10TYCh. 20 - Which of the following is needed for sequencing...Ch. 20 - A tiny amount of blood was recovered from a crime...Ch. 20 - Which technique will allow amplification of the...Ch. 20 - A gene for a bacterial protein with insecticide...Ch. 20 - Prob. 15TYCh. 20 -
1. What do you think are the chances that someone...Ch. 20 - Imagine a future in which the respiratory effects...Ch. 20 - Prob. 3AWKCh. 20 - Prob. 4AWKCh. 20 - Prob. 5AWKCh. 20 - There is interest in producing vaccines in foods....Ch. 20 - Transgenic plants have the potential to improve...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Match each enzyme name in the left column with the correct descriptive phrase in the right column. a. Topoisomerase II b. DNA ligase c. DNA polymerase y d. Reverse transcriptase i. Catalyzes most nucleotide incorporations in bacterial DNA replication ii. Cleaves RNA in a DNA-RNA hybrid molecule e. DNA polymerase I f. DNA polymerase II iii. Uses a tRNA primer in synthesis of retroviral DNA iv. Acts through an adenylylated DNA inter- mediate v. Catalyzes formation of a double-strand DNA break vi. Catalyzes mitochondrial DNA replicationarrow_forwardWhen E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as : A. Ligation. B. Transformation. C. Transfection. D. Digestion.arrow_forwardWhich of the following molecules helps relieve the tension of unwinding parental DNA strands, by breaking DNA and rejoining it before it is replicated? a. Primase b. Single-stranded binding proteins c. Helicase d. Topoisomerasearrow_forward
- Plasmids are important in biotechnology because they are Group of answer choices a. a vehicle for the insertion of foreign genes into bacteria b. recognition sites on recombinant DNA strands c. surfaces for protein synthesis in eukaryotic recombinants d. viruses incorporated into the host DNAarrow_forwardWhich of the following objects would you use microinjection in order to transfer DNA into? a. Egg cell visible to the human eye b. Animal virions c. Escherichia coli that has an F plasmid d. Bacteria that has been heat shockedarrow_forwardExplain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forward
- Choose the combination of answers that most accurately completes the statement.Which of the following is not essential to carry out the polymerase chain reaction? a. primers c. gel electrophoresis b. DNA polymerase d. high temperaturearrow_forwardWhich of the following enzymes is responsible for ensuring that genetic material at the end of the chromosome is not lost during DNA replication? a. Helicase b. DNA Polymerase c. Telomerase d. DNA Ligase e. Primasearrow_forwardWhy must a genetically engineered plasmid contain a genetic marker? a.) to prevent the construction of an artificial chromosome b.) to separate cells that contain recombinant DNA from those that do not c.) to produce multiple copies of the recombined plasmid after heat treatment d.) to break apart the circular plasmid and introduce another DNA fragmentarrow_forward
- The general excision repair pathway for DNA repair has the following order A. endonuclease cuts → DNA ligase removes → endonuclease removes → DNA ligase B. DNA ligase → DNA polymerase → helicase or endonuclease removes → endonuclease cuts C. endonuclease cuts → helicase or endonuclease removes → DNA polymerase → DNA ligase D. endonuclease cuts → DNA polymerase repairs → helicase opens up → DNA ligasearrow_forwardWhich statement about Okazaki fragments is true? Select one: a. DNA polymerase doesn’t need a primer to build these fragments b. They act as a primer that initiates DNA replication. c. They correct errors made during earlier phases of DNA replication. d. They are necessary because DNA polymerase can only build DNA in the 5’ to 3’ direction, so for one of the strands at each fork, the DNA polymerase can only buildaway from the fork. e. They prevent the ends of chromosomes from shortening with every replication.arrow_forwardRecombinant DNA molecules are produced by incubating plasmids that have been broken open by a restriction enzyme together with DNA molecules containing complementary sticky ends in the presence of the enzyme ? 1) exonuclease 2) DNA connectase O 3) DNA ligase 4) DNA polymerase O 5) endonucleasearrow_forward
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