Biology: Life on Earth with Physiology (11th Edition)
11th Edition
ISBN: 9780133923001
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Textbook Question
Chapter 20, Problem 2RQ
What are nitrogen-fixing bacteria, and what role do they play in ecosystems?
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 20 Solutions
Biology: Life on Earth with Physiology (11th Edition)
Ch. 20.1 - describe some differences between bacteria and...Ch. 20.1 - describe the typical sizes and shapes of...Ch. 20.2 - Unwelcome Dinner Guests A few of the bacteria that...Ch. 20.2 - describe the range of environments inhabited by...Ch. 20.2 - As part of a study on the relationship between...Ch. 20.2 - Unpleasant breath odors are caused mainly by...Ch. 20.2 - What might explain the observation that most...Ch. 20.2 - describe adaptations that help protect prokaryotes...Ch. 20.2 - Some of the enzymes that have important uses in...Ch. 20.2 - explain how prokaryotes reproduce and exchange...
Ch. 20.2 - What is the main advantage of prokaryotic fission,...Ch. 20.3 - Unwelcome Dinner Guests Many of the bacteria...Ch. 20.3 - explain how prokaryotes affect animal and plant...Ch. 20.3 - If all of Earths nitrogen-fixing prokaryotes were...Ch. 20.3 - explain prokaryotes role in nutrient recycling?Ch. 20.3 - describe how prokaryotes help clean up pollution?Ch. 20.3 - describe some of the pathogenic bacteria that...Ch. 20.4 - Unwelcome Dinner Guests How do harmful bacteria...Ch. 20.4 - describe the structure and characteristics of...Ch. 20.4 - Why are viruses unable to replicate outside of a...Ch. 20.4 - describe the effects they can have on host...Ch. 20.4 - Biotechnologists often use viruses to transfer...Ch. 20 - Prob. 1ACCh. 20 - ____________, have peptidoglycan in...Ch. 20 - The name of the process by which DNA is...Ch. 20 - Describe some of the ways in which prokaryotes...Ch. 20 - Before the discovery of prions, many (perhaps...Ch. 20 - Prokaryotic cells are ____________,...Ch. 20 - A community of prokaryotes surrounded by slime and...Ch. 20 - What are nitrogen-fixing bacteria, and what role...Ch. 20 - Many prokaryotes use ____________ to move about....Ch. 20 - Which of the following statements about archaea is...Ch. 20 - Describe some of the extreme environments in which...Ch. 20 - ____________,bacteria inhabit environments that...Ch. 20 - Viruses a. are usually photosynthetic. b. consist...Ch. 20 - What is an endospore? What is its function?Ch. 20 - Prokaryotes reproduce by ____________ and may...Ch. 20 - Applying fertilizer near an oil spill to Increase...Ch. 20 - What is conjugation? What role do plasmids play in...Ch. 20 - The plant nutrient ammonium is produced by...Ch. 20 - Why are prokaryotes especially useful in...Ch. 20 - Cholera, gonorrhea, and pneumonia are some of the...Ch. 20 - Describe the structure of a typical virus. How do...Ch. 20 - A virus consists of a molecule of __________ or...Ch. 20 - Describe some examples of how prokaryotes are...Ch. 20 - How do archaea and bacteria differ? How do...
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forward
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