Microbiology: A Systems Approach
Microbiology: A Systems Approach
4th Edition
ISBN: 9780073402437
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 20, Problem 2CC
Summary Introduction

To determine:

The vector-borne diseases those are bacterial.

Introduction:

Vectors are the mean to transmit the diseases from one infected person, or from one animal to another. Bacteria are defined as small, microscopic, prokaryotic and unicellular organisms that can be pathogenic. Diseases which are caused by bacteria are termed as bacterial diseases.

Summary Introduction

To determine:

The vector-borne diseases that are protozoan

Introduction:

Vectors are the means to transmit diseases from one infected person or from one animal to another. The disease or infection which is caused by protozoa is called as protozoal disease.

Summary Introduction

To determine:

The vector-borne diseases those are viral.

Introduction:

Vectors are the means to transmit the diseases from one infected person or from one animal to another. The disease or infection which is caused by virus is called viral disease. Virus affects organisms by attaching to the body and enters inside it. This is followed by replication of virus particle

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a) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following:  Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng  Final recovery volume: 0.50 mL  Volume plated: 0.25 mL  Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) b) Using the equation above, calculate the transformation efficiency. c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?   b) Predict the growth you would expect to see on each of the following plates:         – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?  2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following:  Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng  Final recovery volume: 0.50 mL  Volume plated: 0.25 mL  Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
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