Microbiology: A Systems Approach
4th Edition
ISBN: 9780073402437
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Question
Chapter 20.3, Problem 12AYP
Summary Introduction
To explain:
The rationale behind the recommended treatment for HIV infection.
Concept introduction:
Virus is an infectious small particle that can’t be seen through the naked eyes. The human immunodeficiency virus is a lentivirus which is a causative agent of AIDS. This virus attacks T-cells, that provide immunity against the infection. The decline in number of T cells results in loss of the immunity and individual becomes susceptible to the virus. HIV enzyme, reverse transcriptase transcribes the viral genetic material RNA to DNA that can actively incorporate itself into the genetic material of the host causing AIDS.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
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Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 20 Solutions
Microbiology: A Systems Approach
Ch. 20.1 - Describe the important anatomical features of the...Ch. 20.1 - List the natural defenses present in the...Ch. 20.2 - Prob. 3AYPCh. 20.3 - Prob. 2CFCh. 20.3 - Prob. 4AYPCh. 20.3 - Prob. 5AYPCh. 20.3 - Prob. 6AYPCh. 20.3 - Prob. 7AYPCh. 20.3 - Prob. 8AYPCh. 20.3 - Prob. 9AYP
Ch. 20.3 - Prob. 10AYPCh. 20.3 - Prob. 11AYPCh. 20.3 - Prob. 12AYPCh. 20.3 - Prob. 13AYPCh. 20 - Prob. 1CFCh. 20 - Prob. 1MCQCh. 20 - Prob. 2MCQCh. 20 - Prob. 3MCQCh. 20 - Rabbit fever is caused by a. Yersinia pestis. b....Ch. 20 - Prob. 5MCQCh. 20 - Prob. 6MCQCh. 20 - Prob. 7MCQCh. 20 - Prob. 8MCQCh. 20 - Prob. 9MCQCh. 20 - Prob. 10MCQCh. 20 - Brucellosis can be transmitted to humans by...Ch. 20 - Prob. 12TFCh. 20 - Prob. 13TFCh. 20 - Prob. 14TFCh. 20 - Prob. 15TFCh. 20 - Prob. 1CTQCh. 20 - Explain why cases of dengue fever have been...Ch. 20 - Prob. 3CTQCh. 20 - Prob. 4CTQCh. 20 - Prob. 5CTQCh. 20 - Prob. 6CTQCh. 20 - Prob. 7CTQCh. 20 - Prob. 8CTQCh. 20 - Prob. 9CTQCh. 20 - Prob. 10CTQCh. 20 - Prob. 1CCCh. 20 - Prob. 2CCCh. 20 - Prob. 3CCCh. 20 - Prob. 4CCCh. 20 - Prob. 1VCCh. 20 - Prob. 1CM
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