Concept explainers
The water in the Yellowstone National Park hot springs shown here is around 160°F (70°C). Biologists assumed that no species of organismscould live in water above about 130°F(55*C), so thev were surprised to find several species of bacteria there, now called thermophiles
15. SYNTHESIZE YOUR KNOWLEDGE |
("heat-lovers"). You've learned in this chapter how an enzyme from one species, Thermus aquaticus, made feasible one of the most important DNA-based techniques used in labs today. ldentify the enzyme, and indicate the value of its being isolated from a thermophile. Suggest other reasons why enzymes from this bacterium (or other therrnophiles) might also be valuable.
Want to see the full answer?
Check out a sample textbook solutionChapter 20 Solutions
Campbell Biology: Custom Edition
Additional Science Textbook Solutions
Laboratory Manual For Human Anatomy & Physiology
SEELEY'S ANATOMY+PHYSIOLOGY
Biological Science (6th Edition)
Biology: Life on Earth with Physiology (11th Edition)
Campbell Biology in Focus (2nd Edition)
- In the table below designate growth (G) or no growth (NG) for each organism. Organism TSA Plates Aerobic Plate Anaerobic Plate Escherichia coli Pseudomonas aeruginosa Clostridium sporogenes (arowtn NO Grawtn 2. How would the following organisms be classified in terms of aerotolerance (from your results)? Explain how the results you recorded above support the classification. E. coli ESCnericnia coli is a FacuItative anacrobe bicause it canaoin the presence or awsine of 02 P. oeruginosa Psudomonas aeruginosa is a obigatn aoloe because It Piquires Oz For groNth C sporogenes CI 0STVI dium sporogenes is a obligare anaeobe blcauss it in the presence oF O Cannot arow 3. What region of the TSA tube is anaerobic? Aerobic? What part of the FTM tube is anaerobic? How do you know? 4. What is used to make the Anaerobic chamber anaerobic? PrOVides an anaurobIc environment oy Usin Onaunbe gas p0CK produces H, and cOzgases,and has sidi poUcn Ith metai CataNST (pall 5. What is the purpose of the dye…arrow_forward-n f estion B I ut of question PF budy As you increase the amount of substrate in a reaction (while keeping the enzyme concentration the same): Select one: O A. The amount of products formed should decrease OB. The amount of products formed should remain the same OC. The amount of products formed should increase Clear my choice Mixing hydrogen peroxide with an enzyme different than catalase (such as lactase) should also result in the formation of products. Select one: O True O False The temperature at which an enzyme works best can differ from enzyme to enzyme. Select one: O True False B 7 Oarrow_forwardThe covalent catalytic mechanism of an enzyme depends on a single active site Cys whose pKa is 8. A mutation in a nearby residue alters the microenvironment so that this pKa increases to 10. Would the mutation cause the reaction rate to increase or decrease? Justify your answer.arrow_forward
- When the rate of reaction decreases, what is happening to the enzyme, catalase? Did your actual results match your hypotheses? If not, why? Is the optimum temperature the same for all enzymes? Explain.arrow_forwardPart 2 must be done at a constant temperature. What steps were taken in the experiment to satisfy this requirement?arrow_forwardPlease also give example of the bacteriaarrow_forward
- The thermoacidophile Sulfolobus acidocaldarius is an archaean organism that can tolerate environments that are high in temperature and low in pH. It lacks peptidoglycan, but still possesses a cell wall. Which of the following statements is likely to be an accurate description of this species? A. It is a bacterium. B. The optimal pH of its enzymes will lie above pH 7. C. It could inhabit hydrothermal springs. D. It could inhabit alkaline hot springs.arrow_forwardConsider the Michaelis-Menten enzymes below and answer the following questions. Kcat (s') 9.5*105 1.4*10* 2.5*102 1.0*107 5.0*10 8.0*10² Enzyme Km (M) A В a. Which enzyme has the highest affinity substrate? How do you know? b. Which enzyme can convert the most substrate to product in a given period of time? How do you know? c. Which enzyme has the highest catalytic efficiency? How do you know?arrow_forward1. Carols first question was, “What is an enzyme” a. Use the enzyme lactase as an example, and explain how it functions(figure 4.1) b. What is the substrate for lactase. c. What are the end products of this reaction?arrow_forward
- Biology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage Learning