Concept explainers
(a)
To identify: The genes expressed in a specific type of brain cell.
Introduction:
A particular gene can be identified through its expression in a particular cell and its interaction with other genes. To study the gene expression, specific mRNAs corresponding to a gene of interest are to be identified and reverse transcribed to form cDNA. This sequence can be identified through RT-PCR, DNA microarray analysis, and RNA sequencing. To study a particular protein, multiple copies of the gene fragment encoding that protein are synthesized through polymerase chain reaction (PCR). These gene fragments are then inserted into the expression vector to synthesize a large quantity of proteins encoded by the gene in a process known as gene cloning.
(b)
To isolate: The neurotransmitter gene expressed in brain cells.
Introduction:
A particular gene can be identified through its expression in a particular cell and its interaction with other genes. To study the gene expression, specific mRNAs corresponding to a gene of interest are to be identified and reverse transcribed to form cDNA. This sequence can be identified through RT-PCR, DNA microarray analysis, and RNA sequencing. To study a particular protein, multiple copies of the gene fragment encoding that protein are synthesized through polymerase chain reaction (PCR). These gene fragments are then inserted into the expression vector to synthesize a large quantity of proteins encoded by the gene in a process known as gene cloning.
(c)
To produce: Multiple copies of the gene encoding for neurotransmitter protein.
Introduction:
A particular gene can be identified through its expression in a particular cell and its interaction with other genes. To study the gene expression, specific mRNAs corresponding to a gene of interest are to be identified and reverse transcribed to form cDNA. This sequence can be identified through RT-PCR, DNA microarray analysis, and RNA sequencing. To study a particular protein, multiple copies of the gene fragment encoding that protein are synthesized through polymerase chain reaction (PCR). These gene fragments are then inserted into the expression vector to synthesize a large quantity of proteins encoded by the gene in a process known as gene cloning.
(d)
To produce: Large quantities of the neurotransmitter.
Introduction:
A particular gene can be identified through its expression in a particular cell and its interaction with other genes. To study the gene expression, specific mRNAs corresponding to a gene of interest are to be identified and reverse transcribed to form cDNA. This sequence can be identified through RT-PCR, DNA microarray analysis, and RNA sequencing. To study a particular protein, multiple copies of the gene fragment encoding that protein are synthesized through polymerase chain reaction (PCR). These gene fragments are then inserted into the expression vector to synthesize a large quantity of proteins encoded by the gene in a process known as gene cloning.
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Chapter 20 Solutions
Campbell Biology: Australian And New Zealand Edition + Mastering Biology With Etext
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