Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781259709227
Author: Marjorie Kelly Cowan Professor, Heidi Smith
Publisher: McGraw-Hill Education
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Question
Chapter 2, Problem 5Q
Summary Introduction
To determine:
Whether the functions of microorganisms can be revealed with the help of microscopy techniques
Concept introduction:
Microscopy is the technique which is used to identify cells and microbes under the microscopes. Various structures and functions can be observed with the help of microscopes.
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 2 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 2.1 - Explain what the Five Is are and what each step...Ch. 2.1 - Discuss three physical states of media and when...Ch. 2.1 - Compare and contrast selective and differential...Ch. 2.1 - Provide brief definitions for defined media and...Ch. 2.1 - Medical Moment The Making of the Flu Vaccine: An...Ch. 2.1 - Prob. 1NPCh. 2.1 - Prob. 2NPCh. 2.2 - Prob. 5AYPCh. 2.2 - Prob. 6AYPCh. 2.2 - Prob. 7AYP
Ch. 2.2 - Give examples of simple, differential, and special...Ch. 2.2 - Prob. 3NPCh. 2.2 - Medical Moment Gram-Positive Versus Gram-Negative...Ch. 2 - The identities of microorganisms on our planet a....Ch. 2 - Prob. 2QCh. 2 - Often bacteria that are freshly isolated from a...Ch. 2 - Which of these types of organisms is least likely...Ch. 2 - Prob. 5QCh. 2 - Some bacteria can produce a structure called an...Ch. 2 - A fastidious organism must be grown on what type...Ch. 2 - Write a short paragraph to differentiate among the...Ch. 2 - Prob. 9QCh. 2 - Viruses are commonly grown in/on a. animal cells...Ch. 2 - Can you devise a growth medium with ingredients...Ch. 2 - There is a type of differential medium that can...Ch. 2 - Prob. 13QCh. 2 - Several bacteria live naturally in a material on...Ch. 2 - Archaea often grow naturally in extreme...Ch. 2 - Prob. 16QCh. 2 - After performing the streak plate procedure on a...Ch. 2 - You are a scientist studying a marsh area...Ch. 2 - Prob. 19QCh. 2 - Prob. 20QCh. 2 - You perform the special stain for bacterial...Ch. 2 - Prob. 1VC
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