Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781259709227
Author: Marjorie Kelly Cowan Professor, Heidi Smith
Publisher: McGraw-Hill Education
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Chapter 2, Problem 19Q
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Which of the following describes the usefulness of a Gram stain. Select all that apply.
a. A Gram stain can be used to check for bacteria at the site of a suspected infection or in certain body fluids, such as blood or urine.
b. A Gram stain can be used to determine whether penicillin will be effective at treating the patient or whether a different antibiotic should be used.
c. A Gram stain can determine whether the bacteria have a thick or thin layer of peptidoglycan in their cell wall.
Which of the following can be used to distinguish one colony from another on an agar plate? Select all that apply.
a. Shape
b. Color
c. Gram stain
d. Cell arrangement
e. Size
f. Elevation
g. Margin
h. Texture
Explain how bacterial cells would look in the gram staining procedure if the following mistakes were made:
a. Decolorizer left on too long
b. Decolorizer not left on long enough
c. Slide not heat-fixed before staining
Chapter 2 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 2.1 - Explain what the Five Is are and what each step...Ch. 2.1 - Discuss three physical states of media and when...Ch. 2.1 - Compare and contrast selective and differential...Ch. 2.1 - Provide brief definitions for defined media and...Ch. 2.1 - Medical Moment The Making of the Flu Vaccine: An...Ch. 2.1 - Prob. 1NPCh. 2.1 - Prob. 2NPCh. 2.2 - Prob. 5AYPCh. 2.2 - Prob. 6AYPCh. 2.2 - Prob. 7AYP
Ch. 2.2 - Give examples of simple, differential, and special...Ch. 2.2 - Prob. 3NPCh. 2.2 - Medical Moment Gram-Positive Versus Gram-Negative...Ch. 2 - The identities of microorganisms on our planet a....Ch. 2 - Prob. 2QCh. 2 - Often bacteria that are freshly isolated from a...Ch. 2 - Which of these types of organisms is least likely...Ch. 2 - Prob. 5QCh. 2 - Some bacteria can produce a structure called an...Ch. 2 - A fastidious organism must be grown on what type...Ch. 2 - Write a short paragraph to differentiate among the...Ch. 2 - Prob. 9QCh. 2 - Viruses are commonly grown in/on a. animal cells...Ch. 2 - Can you devise a growth medium with ingredients...Ch. 2 - There is a type of differential medium that can...Ch. 2 - Prob. 13QCh. 2 - Several bacteria live naturally in a material on...Ch. 2 - Archaea often grow naturally in extreme...Ch. 2 - Prob. 16QCh. 2 - After performing the streak plate procedure on a...Ch. 2 - You are a scientist studying a marsh area...Ch. 2 - Prob. 19QCh. 2 - Prob. 20QCh. 2 - You perform the special stain for bacterial...Ch. 2 - Prob. 1VC
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- The bacterium Bacillus anthracis was stained with gram staining, endospore staining, acid-stain staining and capsule staining. What result would you expect to see? a. none of these answers b. gram positive, endospore negative, acid-fast negative, capsule positive c. gram negative, endospore positive, acid-fast positive, capsule negative d. gram positive, endospore positive, acid-fast negative, capsule positive e. gram negative, endospore positive, acid-fast negative, capsule negative thank you!!arrow_forwardPut the numbers in the space provided to indicate the order of Capsule Staining. If the statement is not part of the process, put 0. a. Put a loopful of bacteria to the slide. b. Air dry then heat fix the slide. c. Add crystal violet to the slide. d. Add congo red to the slide. e. Apply ethanol.arrow_forwardGive at least one disease that can be diagnosed by: a. Gram Stain b. Acid-fast stain c. Endospore stain d. Capsule stain e. Flagella Stainarrow_forward
- Which of the following acts as the decolorizer in the gram staining? A. Safranine B. Iodine C. Ethanol D. Crystal Violetarrow_forwardWhat method is best for for isolating a colony on a TSA plate? A. Swab plate B. Straight line inoculation with a loop C. Streak plate with a loop D. Spread plate with a glass spreaderarrow_forwardWhich one of the following is the most resistant microorganism for sterilization: Select one: a. Lipid enveloped viruses b. Gram negative bacteria c. Gram positive bacteria d. Mycobacteriumarrow_forward
- observe the transparent bacteria under bright field microscope, we use a. Simple staining b. Gram staining c. Negative staining d Direct staining eAll the abovearrow_forwardThis agar plate was obtained by spreading 0.1 ml of a 1:1,000 dilution of a bacterial sample taken from cole slaw, and then incubating at 37oC for 24h. This plate indicates that: a. there are 42 bacteria/ml on the cole slaw b. there are at least 4 different types of bacteria in the cole slaw c. there are approximately 42,000 bacteria/ml in the cole slaw sample d. there are approximately 420,000 bacteria/ml in the cole slaw e. these bacteria cannot cause illness because they came from the person’s skin who made the cole slawarrow_forwardYou make a smear of E. coli and then simple stain the slide with methylene blue. If you failed to use correct aseptic technique by not sterilizing the loop before picking up the bacterial sample for the smear, what results may be seen under the microscope? A Both blue and purple cells may be seen on the slide. B No cells may be visible on the slide. C There may be cells of different shapes, sizes, and arrangements.arrow_forward
- What type of medium is best used to maintain a preserve specimen before clinical analysis? A. Enriched b. Selective c. Differential d. Reducing e. Nutrientarrow_forwardAmong the many methods, please identify Bacillus cereus bacteria, in staining reactiontests, such as A.simple staining B.gram staining C. isolation tests To distinguish specific cultural characteristics of Bacillus cereus bacteria, please explain how to identify Bacillus cereus in these tests below? D indole test, E.starchhydrolysis test F. citrate test.arrow_forwardWhat is the purpose of diluting a culture by making "quadrant streaks" (or "streak plating") bacteria, and what can we use them for? Check all that apply A. To observe colony morphology/color B. To isolate viral plaques C. testing growth conditions or treatments (such as media, antibiotics, incubation temperature etc.,) D. To isolate a clonal population of bacteria from the rest of the culture E. For subculturing (transferring to new media so we can grow more bacteria, storing the plate in a fridge for repeated use so the bacteria don't die quickly) F. Accurate quantification (to calculate CFU/mL) G. To know what strain of bacteria you're working with, since you can tell based on how it looks on the plate. H. To lower risk of contamination/identify contaminationarrow_forward
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