Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 2, Problem 27P
Interpretation Introduction
Interpretation:
The reason why the definite groups in the folded proteins have about more than 500 pKavalues should be determined.
Concept introduction:
A peptide bond exists between the two amino acids. During the formation of a peptide bond, a molecule of water is released. The carboxyl group of one amino acid gets linked with the amino group of the other amino acid. Polypeptides and proteins are the chains formed by the amino acids.
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Students have asked these similar questions
. A protein gives, under conditions of buffer composition, pH, and tem-
perature that are close to physiological conditions, a molecular weight
by sedimentation equilibrium measurements of 140,000 g/mol. When
the same protein is studied by SDS gel electrophoresis in the absence
or presence of the reducing agent B-mercaptoethanol (BME), the pat-
terns seen, respectively, in lanes A and B are observed. Lane C contains
standards of molecular weight indicated. From these data, describe the
native protein, in terms of the kinds of subunits present, the stoi-
chiometry of subunits, and the kinds of bonding (covalent, noncova-
lent) existing between subunits. /
A
B
- BME
+ BME
STD
Serum albumin,
67,000
Ovalbumin,
43,000
Carbonic anhydrase,
30,000
Trypsin inhibitor,
20,000
10
2.
4.
Co
cm migrated
need help to calculate P1 for amino acid Tryptophan. Also please check if I draw the mechanism correctly and completely？？？
Predict the number of bands and apparent mol. wt. of the following proteins on SDS gels.
1. A trimeric protein containing three chains, each with a molecular weight of 60,000 Da (60 kDa).
Chapter 2 Solutions
Biochemistry
Ch. 2 - Prob. 1PCh. 2 - Prob. 2PCh. 2 - Prob. 3PCh. 2 - Prob. 4PCh. 2 - Prob. 5PCh. 2 - Prob. 6PCh. 2 - Prob. 7PCh. 2 - Prob. 8PCh. 2 - Prob. 9PCh. 2 - Prob. 10P
Ch. 2 - Prob. 11PCh. 2 - Prob. 12PCh. 2 - Prob. 13PCh. 2 - Prob. 14PCh. 2 - Prob. 15PCh. 2 - Prob. 16PCh. 2 - Prob. 17PCh. 2 - Prob. 18PCh. 2 - Prob. 19PCh. 2 - Prob. 20PCh. 2 - Prob. 21PCh. 2 - Prob. 22PCh. 2 - Prob. 23PCh. 2 - Prob. 24PCh. 2 - Prob. 25PCh. 2 - Prob. 26PCh. 2 - Prob. 27PCh. 2 - Prob. 28PCh. 2 - Prob. 29PCh. 2 - Prob. 30PCh. 2 - Prob. 31PCh. 2 - Prob. 32PCh. 2 - Prob. 33PCh. 2 - Prob. 34PCh. 2 - Prob. 35PCh. 2 - Prob. 36PCh. 2 - Prob. 37P
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