Biochemistry: Concepts and Connections
1st Edition
ISBN: 9780321839923
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Textbook Question
Chapter 2, Problem 17P
A student is carrying out a biological preparation that requires 1 M NaCl to maintain an ionic strength of 1.0. The student chooses to use 1.0 M ammonium sulfate instead. Why is this a serious error?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
The glass electrode used in commercial pH meters gives an electrical response proportional to the concentration of hydrogen ion. To convert these responses to a pH reading, the electrode must be calibrated against standard solutions of known H+ concentration. Determine the weight in grams of sodium dihydrogen phosphate (NaH2PO4 · H2O; FW 138) and disodium hydrogen phosphate (Na2HPO4 ; FW 142) needed to prepare 1 L of a standard buffer at pH 7.00 with a total phosphate concentration of 0.100 M . See Problem 24 for the pKa values of phosphoric acid.
If a pharmacist needs to prepare a bacterial suspension with a concentration of 2.4x10^2 cfu using 1 mL of 2.4 x 10^4 bacterial suspension, what size of volumetric flask will she/he be needing?
The pH probe/meter uses following equations:
Ecell = L + 0.0592 log a1 = L - 0.0592 pH
Where L = L1 + EAg/AgCI + Easy= constants
L1 = - 0.0592 log a2
a1 = activity of analyte solution a2 = activity of internal solution
How will measured pH value be affected vs “real” pH if HCl in pH electrode, became 0.15M instead of 0.1M ?
a.impact can not be determined
b.measured pH is higher than "real" pH.
c.measured pH is lower than "real" pH.
d.measured pH is same as "real" pH.
Chapter 2 Solutions
Biochemistry: Concepts and Connections
Ch. 2 - Suppose a chloride ion and a sodium ion are...Ch. 2 - Draw two different possible hydrogen-bonding...Ch. 2 - Prob. 3PCh. 2 - 4. What is the pH of each of the following...Ch. 2 - Prob. 5PCh. 2 - The weak acid HA is 2% ionized (dissociated) in a...Ch. 2 - 7. Calculate the pH values and draw the titration...Ch. 2 - What is the pH of the following buffer mixtures?...Ch. 2 - a. Suppose you wanted to make a buffer of exactly...Ch. 2 - Prob. 10P
Ch. 2 - You need to make a buffer whose pH is 7.0, and you...Ch. 2 - Describe the preparation of 2.00 L of 100 glycine...Ch. 2 - Carbon dioxide is dissolved in blood (pH 7.4) to...Ch. 2 - What is the molecular basis for the observation...Ch. 2 - The anno acid arginine ionizes according to the...Ch. 2 - It is possible to make a buffer that functions...Ch. 2 - A student is carrying out a biological preparation...Ch. 2 - Histidine is an amino acid with three titratable...Ch. 2 - Prob. 19PCh. 2 - Prob. 20PCh. 2 - Prob. 21P
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- What is the minimum volume of dissolution media required to provide sink condition in dissolution test of a tablet dosage form (its weight: 1350 mg; dose strength: 1000 mg) regarding the following conditions? The molecular weight of an active drug substance is 500 Da; the dissolution media is water and the solubility of active drug substance in water is 10 mg/Ml (4. SLAYT) 500 ml 1000 ml 1350 ml 100 ml 135 mlarrow_forwardHow much of the enzyme proteinase k (solute) is required to make 250ml of a solution with a concentration of .01mg/ml? (Weight/volume ratio)arrow_forwardThe pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if the temperature of the sample is 30C when pH was measured ? a.measured pH is lower than real pH b.impact can not be determined c.measured pH is higher than real pH d.measured pH is same as real pHarrow_forward
- The isoelectric point of eIF4a is 5.02. Students are given a sample of cell lysate containing eIF4A at pH 7.4. They are also provided with two buffers of pH 7.4 to use for ion exchange chromatography. One buffer has a very high salt concentration (1 M), the other has a low salt concentration (0.1 M). Mariela decides to use an anion exchange column, while Ashok chooses a cation exchange column. Who made the better decision? Provide a detailed explanation of why one student will end up with a purer sample of eIF4A. Which buffer will you use while washing away impurities (high or low salt), and which would you use to remove eIF4A from the column? Explain your choice.arrow_forwardThe normal freezing point of benzene (C6H6) is 5.5 °C. If 30.92 grams of the nonvolatile nonelectrolyte diphenylether (C12H100), are dissolved in 212.1 grams of benzene, what is the freezing point of the resulting solution? Kfp for benzene is 5.12 °C/m. °Carrow_forwardA 2.5mg/ml solution of ampicillin is available for use, what volume would youadd to 200ml of LB medium to obtain a final concentration of 50µg/ml ofampicillin?arrow_forward
- Normal arterial pH is 7.35 to 7. 45. Both metabolic and respiratory alkalosis have pH more basic compare to the normal arterial pH. The difference is 0.5 units. What is pH value in alkalosis? Niemann-Pick type C disease is a progressive neurological disease; symptoms include unsteady walking with uncoordinated limb movements, slurred speech, a difficulty in swallowing, epilepsy and tremor. This condition is due to the abnormal functioning of a lysosome. State the normal functions performed by this organelle.arrow_forward10 tab of acetaminophen 500 mg molar absorptivity= 14520 @ 243nm. The total weight of the 10tab is 5278 mg. I take a sample equivalent to 100mg of tablet and I diluted it in 100mL, I took an aliquot of 10mL and I diluted it in 250mL, of these I took 25mL and I diluted them in 250mL. Calculate the weight per tablet. Absorbance = 0.944arrow_forwardplease fill in both blanksarrow_forward
- How much of a 10x buffer should be added to 900ml of water to make a 1x solution?arrow_forwardMatch the common laboratory scenarios to the correct buffer required using the guide below. Molecule pKa Value(s) Acetic acid 4.8 Carbonic acid 6.1, 10.2 Citric acid 3.1, 4.8, 5.4 HEPES 7.5 Phosphoric acid 2.1, 7.1, 12.3 TRIS 8.3 Mammalian cells require a pH of about 7.4 for cell and tissue culture experiments. Elution of remaining molecules from a cation exchange column requires washing with pH 10 buffer. Dissolution of lysozyme requires pH 3 buffer to maintain activity. Elution of remaining molecules from an anion exchange column requires washing with pH 2 buffer.…arrow_forward80.00 mL of 0.350 M benzoic acid (K = 6.4×105) is titrated by 0.350 M NaOH. Calculate the pH of the acid solution before any titrant is added. PH Calculate the pH after 58.9 mL of 0.350 M NaOH is added to 80.00 mL of 0.350 M benzoic acid. pH = Calculate the pH after 80.00 mL of 0.350 M NaOH is added to 80.00 mL of 0.350 M benzoic ad pH = Calculate the pH after 110 mL of 0.350 M NaOH is added to 80.00 mL of 0.350 M benzoic acid. pH=arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
GCSE Chemistry - Acids and Bases #34; Author: Cognito;https://www.youtube.com/watch?v=vt8fB3MFzLk;License: Standard youtube license