Biochemistry: Concepts and Connections
Biochemistry: Concepts and Connections
1st Edition
ISBN: 9780321839923
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
Question
Book Icon
Chapter 2, Problem 19P
Interpretation Introduction

(a) Interpretation:

If the enzyme is more active at pH 3.5 or 4.5 should be explained.

Concept Introduction:

The acidic group in amino acid is protonated at lower pH value and basic group is protonated at high pH value. Thus, for a amino acid, if pKa>pH, acidic or −COOH group is protonated and if pKa<pH then basic group is protonated.

Interpretation Introduction

(b) Interpretation:

The fraction of the enzymes active at pH 4.07 should be determined.

Concept Introduction:

The Henderson-Hasselbalch equation that relates pH and pKa of solution is as follows:

    pH=pKa+log[A][HA]

Here, pH is negative log of hydrogen ion, pKa is negative log of acid dissociation constant, [HA] is concentration of weak acid and [A] is concentration of conjugate base.

Interpretation Introduction

(c) Interpretation:

The pH value at which enzyme show 78% of maximal activity.

Concept Introduction:

The pH of solution can be calculated from Henderson-Hasselbalch equation as follows:

    pH=pKa+log[A][HA]

Here, pH is negative log of hydrogen ion, pKa is negative log of acid dissociation constant, [HA] is concentration of weak acid and [A] is concentration of conjugate base.

Blurred answer
Students have asked these similar questions
1 2 Br OCH₁₂ CF3 3 4 2. Which one shows the correct order of reactivity of these four compounds in electrophilic aromatic substitution reactions, with more reactive > least reactive ? A) 1>2>3>4 B) 13>4> 2 C) 3 > 2 > 1 > 4 D) 4 > 3 > 1 > 2 E) 3 >1>2> 4
12. Which of the compounds below is expected to be the major product formed by this reaction sequence? COOH Br A C₂H5CI H₂CrO4 Br₂ FeBr3 AICI 3 .COOH .COOH COOH CH₂OH B Br Br C D Br E Br
11. Which of these chlorobenzenes does not undergo nucleophilic aromatic substitution upon treatment with NaNH2? CI CI CI CI CI A B C D E
Knowledge Booster
Background pattern image
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
Text book image
Biochemistry
Biochemistry
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:W. H. Freeman
Text book image
Lehninger Principles of Biochemistry
Biochemistry
ISBN:9781464126116
Author:David L. Nelson, Michael M. Cox
Publisher:W. H. Freeman
Text book image
Fundamentals of Biochemistry: Life at the Molecul...
Biochemistry
ISBN:9781118918401
Author:Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:WILEY
Text book image
Biochemistry
Biochemistry
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Cengage Learning
Text book image
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Text book image
Fundamentals of General, Organic, and Biological ...
Biochemistry
ISBN:9780134015187
Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:PEARSON