EBK BROCK BIOLOGY OF MICROORGANISMS
15th Edition
ISBN: 8220103633352
Author: Stahl
Publisher: PEARSON
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Chapter 19.9, Problem 1CR
Q What are the major advantages of radioisotopic methods in the study of microbial ecology? What type of controls (discuss at least two) would you include in a radioisotopic experiment to show 14CO2 incorporation by phototrophic bacteria or to show
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Which of the following proteins or protein complexes is directly required for the targeting of mitochondrial inner membrane multipass proteins, such as metabolite transporters, whose
signal sequence is normally not cleaved after import?
OA. TIM22
OB. TIM23
C. OXA
OD. Mia40
OE SAM
QUESTION 9
An animal cell has been wounded and has a small rupture in its plasma membrane. Which of the following is more likely to happen next?
OA. The cell rapidly cleaves by cytokinesis.
OB. The rate of receptor-mediati endocytosis is increased.
OC. The rate of exocytosis is increased.
OD. The rate of pinocytosis is increased.
For the a subunit of a trimeric G protein,
A. a G-protein-coupled receptor GPCR) acts as a guanine nucleotide exchange factor (GEF), whereas a regulator of G protein signaling (RGS) can act as a GTPase-activating protein
(GAP).
B. a GPCR acts as a GAP, whereas an RGS can act as a GEF.
C. both a GPCR and an RGS can act as a GEF.
O D. both a GPCR and an RGS can act as a GAP
OE. None of the above.
Chapter 19 Solutions
EBK BROCK BIOLOGY OF MICROORGANISMS
Ch. 19.1 - Describe the enrichment strategy behind...Ch. 19.1 - Why is sulfate (So42) added to a Winogradsky...Ch. 19.1 - What is enrichment bias? How does dilution reduce...Ch. 19.1 - Why do the results of a direct enrichment of an...Ch. 19.2 - What is a pure culture and why is obtaining one...Ch. 19.2 - How does the agar dilution method differ from...Ch. 19.2 - What criteria serve to demonstrate that a culture...Ch. 19.3 - How might you isolate a morphologically unique...Ch. 19.3 - What is meant by high-throughput in culturing...Ch. 19.3 - What feature of high-throughput culturing relieves...
Ch. 19.4 - How does viability staining differ from stains...Ch. 19.4 - What types of environments limit the application...Ch. 19.4 - Why is it incorrect to say that the GFP is a...Ch. 19.4 - Prob. 1CRCh. 19.5 - What structure in the cell is the target for...Ch. 19.5 - FISH and CARD-FISH can be used to reveal different...Ch. 19.5 - Why is CARD-FISH more suitable than FISH for...Ch. 19.6 - What could you conclude from PCR/DGGE analysis of...Ch. 19.6 - What surprising finding has come out of many...Ch. 19.6 - How has next-generation sequencing technology...Ch. 19.6 - QWhich method, ARISA or T-RFLP, would provide more...Ch. 19.7 - Prob. 1MQCh. 19.7 - What are the advantages and disadvantages of...Ch. 19.7 - Why might a microarray be superior to using...Ch. 19.8 - Prob. 1MQCh. 19.8 - How do environmental genomic approaches differ...Ch. 19.8 - Prob. 3MQCh. 19.8 - Prob. 1CRCh. 19.9 - Prob. 1MQCh. 19.9 - If a large pulse of organic matter entered the...Ch. 19.9 - Q What are the major advantages of radioisotopic...Ch. 19.10 - What is the simplest explanation for why lunar...Ch. 19.10 - What is the expected isotopic composition of...Ch. 19.10 - How might exchange of metabolites among members of...Ch. 19.10 - Will autotrophic organisms contain more or less...Ch. 19.11 - How could NanoSIMS be used to identify a...Ch. 19.11 - Prob. 2MQCh. 19.11 - How does MAR-FISH link microbial diversity and...Ch. 19.11 - Q What can MAR-FISH tell you that FISH alone...Ch. 19.12 - How can stable isotope probing reveal the identity...Ch. 19.12 - What key method is required to do genomics on a...Ch. 19.12 - Prob. 3MQCh. 19.12 - How would you use cytometric cell sorting to...Ch. 19 - Design an experiment for measuring the activity of...Ch. 19 - You wish to know whether Archaea exist in a lake...Ch. 19 - Design an experiment to solve the following...Ch. 19 - Design a SIP experiment that would allow you to...
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- A cell expresses a transmembrane protein that is cleaved at the plasma membrane to release an extracellular fragment. The fragment binds to receptor proteins on nearby cells and activates signaling pathways resulting in altered gene expression patterns in the cells. What form of intercellular signaling does this represent? OA. Contact-dependent signaling B. Paracrine signaling OC. Synaptic signaling D. Endocrine signaling E. Autocrine signalingarrow_forwardWhich of the following drive nuclearly encoded proteins import into the mitochondrial matrix? OA. ATP hydrolysis OB. Membrane Potential OC. K+ Ions OD. A and B OE. None of the abovearrow_forwardWhich of the following statements is true regarding Cholesterol import into the cell? OA. It is transported within LDL B. Receptor mediated endocytosis is involved OC. Release of cholesterol occurs in the Lysosome D. All of the above are true statements OE. None of the above are true statementsarrow_forward
- In which of the following schematic drawings of signaling pathways does the activation of the receptor lead to gene expression? Activating and inhibitory steps are indicated with (+) and (-), respectively. OA OB ㅇㅂㅇㅂㅇㅁ (+) Gene Expression Activated Signaling proteins Transcription receptor activator Bl D Gene Expression Gene Expression ㅁㅁㅇ Gene Expression Gene Expressionarrow_forwardWhich of the following events normally activates a GTP-binding protein? OA. GTP hydrolysis by the protein OB. Activation of an upstream GTPase-activating protein OC. Activation of an upstream guanine nucleotide exchange factor OD. Phosphorylation of a bound GDP molecule by an upstream phosphorylase OE. Pi release after GTP hydrolysisarrow_forwardProteins that are synthesized on the Rough ER are imported into the ER lumen after their signal sequence is recognized. Which of the following is responsible for the signal recognition? OA. Signal Recognition Particle, OB. Signal Sequence C. Stop-transfer sequence OD. Both B and C OE. None of the abovearrow_forward
- Stroma Light 4 H 4 H I Thylakoid- membran 2 H₂O D1 D2 Min Light 2,6 ATP 2 NADP+ 2 NADPH/H+ Fp 2,6 ADP +2,6 P 8 H+ 12 H+ 4 H+ Lumen PS II Cyt bef PS I ATP-Synthase 12345 5a 5b 6arrow_forwardWhich of the following statements is TRUE regarding the signal recognition particle (SRP)? OA. SRP is made up of RNA and Proteins B. SRP binds to the signal sequence OC. SRP binds to the elongation factor binding site on the ribosome and pauses translation D. All of the above are true statements OE. None of the above are true statementsarrow_forwardWhich of the following pathways does NOT directly deliver materials to lysosomes? OA. Endocytosis B. Exocytosis OC. Phagocytosis OD. Autophagy OE. Macropinocytosisarrow_forward
- You suspect a protein to be secreted out of a cell. What experiment would you perform to follow the movement of this protein through the secretory pathway? A. Pulse - Chase Experiment OB. Signal Sequence Deletion C. Signal Sequence Mutation OD. All of the above E. None of the abovearrow_forwardA schematic drawing of the secretory and endocytic pathways is presented below. Indicate which component in the drawing (A to J) corresponds to each of the following. Your answer would be a 10-letter string composed of letters A to J only, c.g. HICDJABFGE. nuclear envelope Early endosome Late endosome ER Lysosome A ( ) cis Golgi cisterna () modial Golgi cisterna () trans Golgi cisterna [] cis Golgi network (CGN) () trans Golgi network (TGN) () Secretory Vesicle B C D E CYTOSOL F G H plasma membranearrow_forwardWhich of the following guide transport vesicles to their target membrane? A. Rab monomeric GTPases B. Signal Sequence OC. Heat Shock Proteins OD. All of the above O E. None of the abovearrow_forward
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