Biology: How Life Works - Standalone book
Biology: How Life Works - Standalone book
2nd Edition
ISBN: 9781464126093
Author: James Morris, Daniel Hartl, Andrew Knoll, Robert Lue, Melissa Michael, Andrew Berry, Andrew Biewener, Brian Farrell, N. Michele Holbrook
Publisher: W. H. Freeman
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Chapter 19, Problem 7SA
Summary Introduction

To draw:

The lactose operon in E.coli with proper illustration of the structural genes, and explain the mechanism involved in gene expression in the presence and absence of lactose.

Introduction:

The lac operon is the best illustration of gene regulation which is operated in E. coli. Lac operon contains the structural and regulatory elements. It contains three structural genes which are responsible for transcribing the gene into a polycystronic mRNA. Lactose is the main precursor of this pathway which is metabolized by the enzyme β-galactosidase. Lac operon contains the structural genes, lacZ, lacI, and lacY. lacZ contains the coding sequence for the enzyme β-galactosidase which breakdown the lactose moieties.

lacY has coding sequence for the enzyme permease which locates the lactose from external environment to the cells. lacI controls the regulation of lacZ and lacy.

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