GEN COMBO LOOSELEAF MICROBIOLOGY:A SYSTEMS APPROACH; CONNECT ACCESS CARD
5th Edition
ISBN: 9781260149364
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 19, Problem 1VC
Summary Introduction
To discuss:
The vaccine production strategies that could be used to produce a vaccine against meningococcal meningitis.
Concept introduction:
Meningitis is the inflammation of meninges, three protective layers of the nervous system. Bacteria cause most serious forms of meningitis. It is facilitated by the previous infection with respiratory viruses. Some forms of meningitis are also caused by viruses.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 19 Solutions
GEN COMBO LOOSELEAF MICROBIOLOGY:A SYSTEMS APPROACH; CONNECT ACCESS CARD
Ch. 19.1 - Describe the important anatomical features of the...Ch. 19.1 - Prob. 2AYPCh. 19.2 - Discuss the current state of knowledge regarding...Ch. 19.3 - Prob. 4AYPCh. 19.3 - Prob. 5AYPCh. 19.3 - Discuss important features of meningoencephalitis,...Ch. 19.3 - Prob. 7AYPCh. 19.3 - Prob. 8AYPCh. 19.3 - Prob. 9AYPCh. 19.3 - Prob. 10AYP
Ch. 19 - Prob. 1MCQCh. 19 - Prob. 2MCQCh. 19 - Meningococcal meningitis is caused by a....Ch. 19 - Prob. 4MCQCh. 19 - Prob. 5MCQCh. 19 - Prob. 6MCQCh. 19 - Prob. 7MCQCh. 19 - Prob. 8MCQCh. 19 - Prob. 9MCQCh. 19 - Prob. 10MCQCh. 19 - Prob. 11TFCh. 19 - Prob. 12TFCh. 19 - Prob. 13TFCh. 19 - Prob. 14TFCh. 19 - Prob. 15TFCh. 19 - Prob. 1CTQCh. 19 - Prob. 2CTQCh. 19 - Prob. 3CTQCh. 19 - Prob. 4CTQCh. 19 - Prob. 5CTQCh. 19 - Prob. 1VCCh. 19 - Prob. 1CM
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