PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 2818440045677
Author: WILLEY
Publisher: MCG
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Chapter 19, Problem 1AL
Consider the fact that the use of 16S rRNA sequencing as a taxonomic and phylogenetic tool has resulted in tripling the number of bacterial phyla. Why has the advent of this genetic technique expanded the currently accepted number of microbial phyla?
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In studying Lokiarchaeota, researchers identified eukaryotic signature genes and used this information to better understand the relationship between archaeans and eukaryotes. Many other types of genetic analysis can be used that focus on certain types of
genes. One example of this is multilocus sequence typing (MLST), which focuses on examining genes called housekeeping genes. Microbes encode both housekeeping and pathogenic genes in their genome. Drag the examples of genes to the correct bins to
indicate whether they are housekeeping genes, pathogenic genes, or eukaryotic signature genes.
Place the phrase describing a gene product in the correct bin.
► View Available Hint(s)
peptidoglycan synthesis genes
Housekeeping genes
membrane remodeling
genes
cytoskeletal
genes
membrane lipid
genes
Pathogenic genes
endotoxin genes
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ribosomal genes exotoxin genes
Eukaryotic signature genes
Help
Let's say that you're a scientist trying to analyze newly sequenced bacterial DNA genomes from
an environmental sample of rainforest soil, and your first task is to organize all of the genomes
you find into a phylogenetic tree. If you couldn't use the 16S rRNA gene sequence to create the
tree, which of these alternative genes would work best for your task of creating a tree that works
for all the bacteria in your sample?
The gene for Bacteriochlorophyll A
A gene for an RNA polymerase component protein
The first gene in the histidine biosynthesis pathway
The first gene in the LPS synthesis pathway
I need the answer as soon as possible
Chapter 19 Solutions
PRESCOTT'S MICROBIOLOGY
Ch. 19.1 - What is a natural classification? What microbial...Ch. 19.1 - Prob. 2CCCh. 19.1 - Consider the finding that bacteria capable of...Ch. 19.2 - What is the difference between a microbial species...Ch. 19.2 - Why is it important to have a type strain for each...Ch. 19.2 - The genus Salmonella was once thought to contain...Ch. 19.3 - What are the advantages of using each major group...Ch. 19.3 - Why is rRNA so suitable for determining...Ch. 19.3 - Prob. 3CCCh. 19.4 - Could a phylotype be considered an OTU? What about...
Ch. 19.4 - List the differences between distance-based and...Ch. 19.4 - Prob. 3CCCh. 19.4 - You are building a tree based on 16S rRNA sequence...Ch. 19.4 - Is HGT involved in movement of genes in the core...Ch. 19.5 - On what evidence is this hypothesis based?Ch. 19.5 - Construct a scenario in which each of the...Ch. 19.5 - Define ecotype. Do you think it is necessary to...Ch. 19.5 - What is the difference between the core genome and...Ch. 19.5 - Of the following genes, which do you think are...Ch. 19.5 - Prob. 4CCCh. 19.6 - Prob. 1CCCh. 19.6 - Describe two different situations in which it...Ch. 19 - Prob. 1RCCh. 19 - Prob. 2RCCh. 19 - Prob. 3RCCh. 19 - Prob. 4RCCh. 19 - Prob. 5RCCh. 19 - Prob. 6RCCh. 19 - Consider the fact that the use of 16S rRNA...Ch. 19 - You have recently established a pure culture of a...Ch. 19 - Prob. 3ALCh. 19 - Prob. 4ALCh. 19 - Prob. 5ALCh. 19 - Prob. 6ALCh. 19 - Prob. 7AL
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- The universal phylogenetic tree of life shows the divergence of Bacteria, Archaea, and Eukarya. Studies of Lokiarchaeota help to elucidate these relationships, but the first universal tree of life was constructed by Carl Woese using ribosomal RNA sequences. There are advantages to using different types of nucleic acid for different types of analyses. What is a specific advantage of using small subunit ribosomal RNA (SSU rRNA) gene sequences for phylogenetic analysis compared with DNA sequences? ▸ View Available Hint(s) SSU rRNA sequences accumulate mutations more rapidly than protein-encoding DNA. SSU rRNA is frequently transferred through horizontal gene transfer, whereas DNA sequences are not. Primers are required in DNA sequence analysis, but not for SSU rRNA sequence analysis. Although PCR products can be visualized with SSU rRNA, this is not the case with other forms of nucleic acids. DNA sequences are too highly conserved to work well for sequence analysis; individual species are…arrow_forwardWhat are the 4 characteristics/properties of rRNA genes which make them good molecular chronometers. Why viruses are not found in the tree of life? Why might viable cell cultures be of more use in microbial taxonomy than preserved specimens? Is it possible to provide a formal name for a microorganism that has not been cultivated in isolation? What kind of name might be used if a microorganism is well-characterized but cannot yet be cultivated in isolation?arrow_forwardMetagenomics has revolutionized our understanding of the microbial world by allowing the study of organisms that had been impossible to culture. Understanding the Lokiarchaeota required metagenomics analysis, which has also led to the identification of the eukaryotic signature genes in other environments. The terms below relate to genes and genetic analysis. Drag each term to the correct description. Drag and drop the terms on the left to match the description on the right. ▸ View Available Hint(s) Submit Homologous Monophyletic Metagenomics Orthologs Paralogs and that has a different function (this occurs through gene duplication) : the study of genetic material from an environmental sample Reset : a group of organisms in a phylogeny that have a common ancestor : a gene that is related in different species by being inherited from a common ancestor Help and that has the same function : a gene that is related in different species by being inherited from a common ancestor : a gene that…arrow_forward
- Describe the Theory of Endosymbiosis. a) Briefly define the Endosymbiotic Theory. 2 pts,b) Explain why the Theory of Endosymbiosis is considered a “theory” and not a “hypothesis.” 3 pts,c) Provide a description of eukaryotic and prokaryotic cells, including at least 4 similarities and 4 structural differences. 4 pts,d) Describe the sequence of events with anaerobic ancestral proto-eukaryotes and aerobic prokaryotes that led to the formation of mitochondria and chloroplasts inside eukaryotic cells. (Be sure to define aerobic and anaerobic respiration.) 6 pts,e) Explain how each, the anaerobic ancestral proto-eukaryotes and aerobic prokaryotes, benefited from the relationship. 4 pts,f) Describe 7 pieces of evidence for this scientific theory. Some of this evidence will consist of a list of similarities between mitochondria characteristics and bacteria characteristics. Along with this list of similarities, explain HOW the characteristics are similar. 10 pts,g) The Endosymbiotic Theory…arrow_forwardYou have been tasked with designing a new computer algorithm to identify protein-coding genes in bacterial genomes.a) Name 3 specific criteria/patterns you will use to define whether a given sequence could contain a full-length protein-coding gene, and briefly justify your choices.b) Do you think your algorithm will work well to detect proteins encoded by the archaean Sulfolobus acidocaldarius? Why or why not?c) Do you think your algorithm will work well to detect proteins encoded in the eukaryotic yeast Saccharomyces cerevisiae? Why or why not?arrow_forwardDescribe the relationship between the original bacteria kingdom called monera and the domain called bacteria.arrow_forward
- What problem with the six-kingdom classification system was addressed by the three-domain classification system? How did it address the problem?arrow_forwardExplain why the answer is correct. For eukaryote cells to evolve into new types of eukaryotic organisms, which of the following would one expect to occur? The correct answer is E a) these cells have evolved because they have different DNA because of mutations b) Their endoplasmic reticulum is synthesizing new and/or different proteins. c) These specialized cells activate new genes to synthesize new proteins. d) these organisms have new characteristics because they have acquired new genes. e) all of these are truearrow_forwardBriefly discuss the following topics, including appropriate examples for each:3.1. Genomic fingerprinting for the phylogenetic analysis of bacteria 3.2. Photosynthetic pigments and environmental habitats of green sulphur bacteria3.3. Advantages of phage therapy for bacterial infectionsarrow_forward
- The fact that a prokaryote has the ability to make a new protein that is placed in the cell membrane of the mesosome is important to the process of evolution because this represents a) the mutation of a gene, b) new DNA producing a new genetic variation, c) r RNA using a new type of m RNA, d) the species having a greater degree of genetic variability, e) all are truearrow_forwardWhy did Carl Woese propose the domain Archaea? The domain Bacteria already had too many organisms in its classification. The methanogens were discovered to be a complex organism, between prokaryotic bacteria and eukaryotes, thus it needed its own domain. He discovered that methanogens were too large to be classified as bacteria, but too small to be classified as Eukarya. In analyzing the rRNA of methanogens, he discovered that it was completely distinct from any other bacterial RRNA that had been studied. The DNA of methanogens was discovered to use different nucleotides than those of organisms in Eukarya or Bacteria.arrow_forwardDescribe briefly the perks, disadvantages and use of 16s rRNA genes in taxonomic level of classification of bacteria. Cite the claims to be discussed, only here: https://journals.asm.org/doi/full/10.1128/CMR.17.4.840-862.2004 Create a review format of the task.arrow_forward
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