EBK VISUAL ESSENTIALS OF ANATOMY & PHYS
1st Edition
ISBN: 9780321849588
Author: Nath
Publisher: VST
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Chapter 18.2, Problem 1.6SR
Summary Introduction
To label: The structures of the female productive system indicated as 6 in the given figure.
Introduction: Reproductive system (or genital system) is used for the sexual reproduction. The main organs of female reproductive system include vulva, ovaries, uterine tubes, uterus, and vagina. Uterus is pear shaped small organ that nourishes, remove waste and protect the developing fetus. In addition the uterine muscular contraction expels the fetus during birth.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 18 Solutions
EBK VISUAL ESSENTIALS OF ANATOMY & PHYS
Ch. 18.1 - Prob. 1LOCh. 18.1 - Prob. 2LOCh. 18.1 - Prob. 3LOCh. 18.1 - Prob. 4LOCh. 18.1 - Prob. 5LOCh. 18.1 - Prob. 1.1RCh. 18.1 - Prob. 1.2RCh. 18.1 - Describe the functional anatomy of a typical...Ch. 18.1 - Prob. 2.1RCh. 18.1 - Prob. 2.2R
Ch. 18.1 - Prob. 2.3RCh. 18.1 - Prob. 3.1RCh. 18.1 - Prob. 3.2RCh. 18.1 - Prob. 3.3RCh. 18.1 - Prob. 4.1RCh. 18.1 - Prob. 4.2RCh. 18.1 - Prob. 4.3RCh. 18.1 - Prob. 5.1RCh. 18.1 - Prob. 5.2RCh. 18.1 - Prob. 5.3RCh. 18.1 - Prob. 1.1SRCh. 18.1 - Prob. 1.2SRCh. 18.1 - Prob. 1.3SRCh. 18.1 - Prob. 1.4SRCh. 18.1 - Prob. 1.5SRCh. 18.1 - Prob. 1.6SRCh. 18.1 - Prob. 1.7SRCh. 18.1 - Prob. 1.8SRCh. 18.1 - Prob. 1.9SRCh. 18.1 - Prob. 1.10SRCh. 18.1 - Prob. 1.11SRCh. 18.1 - Prob. 2.1SRCh. 18.1 - Prob. 2.2SRCh. 18.1 - Prob. 2.3SRCh. 18.1 - Prob. 2.4SRCh. 18.1 - Prob. 2.5SRCh. 18.1 - Prob. 2.6SRCh. 18.1 - Prob. 2.7SRCh. 18.1 - Prob. 2.8SRCh. 18.1 - Prob. 2.9SRCh. 18.1 - Prob. 2.10SRCh. 18.1 - Prob. 2.11SRCh. 18.1 - Prob. 2.12SRCh. 18.1 - Prob. 2.13SRCh. 18.1 - Prob. 2.14SRCh. 18.1 - Prob. 3SRCh. 18.2 - Prob. 1LOCh. 18.2 - Prob. 2LOCh. 18.2 - Prob. 3LOCh. 18.2 - Describe the anatomy and functions of the vagina...Ch. 18.2 - Prob. 5LOCh. 18.2 - Prob. 6LOCh. 18.2 - Prob. 7LOCh. 18.2 - Prob. 8LOCh. 18.2 - Prob. 1.1RCh. 18.2 - Prob. 1.2RCh. 18.2 - Prob. 1.3RCh. 18.2 - Prob. 2.1RCh. 18.2 - Prob. 2.2RCh. 18.2 - Prob. 2.3RCh. 18.2 - Prob. 3.1RCh. 18.2 - Prob. 3.2RCh. 18.2 - A. How do recently released secondary oocytes...Ch. 18.2 - Prob. 4.1RCh. 18.2 - Prob. 4.2RCh. 18.2 - Prob. 4.3RCh. 18.2 - Prob. 5.1RCh. 18.2 - Prob. 5.2RCh. 18.2 - Trace the route of milk from its site of...Ch. 18.2 - Prob. 6.1RCh. 18.2 - Prob. 6.2RCh. 18.2 - Prob. 6.3RCh. 18.2 - Prob. 7.1RCh. 18.2 - Prob. 7.2RCh. 18.2 - Prob. 7.3RCh. 18.2 - Prob. 8.1RCh. 18.2 - Prob. 8.2RCh. 18.2 - Prob. 8.3RCh. 18.2 - Prob. 1.1SRCh. 18.2 - Prob. 1.2SRCh. 18.2 - Prob. 1.3SRCh. 18.2 - Prob. 1.4SRCh. 18.2 - Prob. 1.5SRCh. 18.2 - Prob. 1.6SRCh. 18.2 - Prob. 1.7SRCh. 18.2 - Prob. 1.8SRCh. 18.2 - Prob. 1.9SRCh. 18.2 - Prob. 1.10SRCh. 18.2 - Prob. 1.11SRCh. 18.2 - Prob. 1.12SRCh. 18.2 - Prob. 1.13SRCh. 18.2 - Prob. 2.1SRCh. 18.2 - Prob. 2.2SRCh. 18.2 - Prob. 2.3SRCh. 18.2 - Prob. 2.4SRCh. 18.2 - Prob. 2.5SRCh. 18.2 - Prob. 2.6SRCh. 18.2 - Prob. 2.7SRCh. 18.2 - Prob. 2.8SRCh. 18.2 - Prob. 2.9SRCh. 18.2 - Prob. 2.10SRCh. 18.2 - Prob. 2.11SRCh. 18.2 - Prob. 2.12SRCh. 18.2 - Prob. 2.13SRCh. 18.2 - Prob. 2.14SRCh. 18.2 - Prob. 3SRCh. 18 - Prob. 1CRQCh. 18 - How might exercise-induced amenorrhea (absence of...Ch. 18 - Prob. 3CRQCh. 18 - Prob. 4CRQCh. 18 - Prob. 5CRQ
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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