CONNECT ACCESS CARD FOR ANATOMY AND PHYSIOLOGY
8th Edition
ISBN: 9781259880193
Author: SALADIN
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Textbook Question
Chapter 18.1, Problem 1BYGO
Identify at least two each of the transport, protective, and regulatory functions of the circulatory system.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 18 Solutions
CONNECT ACCESS CARD FOR ANATOMY AND PHYSIOLOGY
Ch. 18.1 - Identify at least two each of the transport,...Ch. 18.1 - Prob. 2BYGOCh. 18.1 - List the three major classes of plasma proteins....Ch. 18.1 - Prob. 4BYGOCh. 18.1 - Prob. 5BYGOCh. 18.1 - Prob. 1AYLOCh. 18.1 - Prob. 2AYLOCh. 18.1 - Prob. 3AYLOCh. 18.1 - Prob. 4AYLOCh. 18.1 - Prob. 5AYLO
Ch. 18.1 - Prob. 6AYLOCh. 18.1 - Prob. 7AYLOCh. 18.2 - Describe the size, shapte, and contents of an...Ch. 18.2 - Prob. 7BYGOCh. 18.2 - Prob. 8BYGOCh. 18.2 - Prob. 9BYGOCh. 18.2 - Prob. 10BYGOCh. 18.2 - Prob. 11BYGOCh. 18.2 - Prob. 12BYGOCh. 18.2 - Prob. 1AYLOCh. 18.2 - Prob. 2AYLOCh. 18.2 - Prob. 3AYLOCh. 18.2 - Three ways of quantifying the RBCs and hemoglobin...Ch. 18.2 - Prob. 5AYLOCh. 18.2 - Prob. 6AYLOCh. 18.2 - Prob. 7AYLOCh. 18.2 - Prob. 8AYLOCh. 18.2 - Prob. 9AYLOCh. 18.2 - Prob. 10AYLOCh. 18.2 - Causes and effects of hemoglobin deficiencies and...Ch. 18.3 - Prob. 13BYGOCh. 18.3 - Prob. 14BYGOCh. 18.3 - Prob. 15BYGOCh. 18.3 - Prob. 16BYGOCh. 18.3 - Prob. 1AYLOCh. 18.3 - Prob. 2AYLOCh. 18.3 - Prob. 3AYLOCh. 18.3 - Prob. 4AYLOCh. 18.3 - Prob. 5AYLOCh. 18.3 - Prob. 6AYLOCh. 18.3 - Hemolytic disease of the newborn; why it seldom...Ch. 18.4 - Prob. 17BYGOCh. 18.4 - Prob. 18BYGOCh. 18.4 - Prob. 19BYGOCh. 18.4 - Prob. 20BYGOCh. 18.4 - Prob. 21BYGOCh. 18.4 - Prob. 1AYLOCh. 18.4 - Prob. 2AYLOCh. 18.4 - Prob. 3AYLOCh. 18.4 - Prob. 4AYLOCh. 18.4 - Prob. 5AYLOCh. 18.4 - Prob. 6AYLOCh. 18.4 - Prob. 7AYLOCh. 18.5 - Prob. 22BYGOCh. 18.5 - Prob. 23BYGOCh. 18.5 - Prob. 24BYGOCh. 18.5 - Prob. 25BYGOCh. 18.5 - Prob. 26BYGOCh. 18.5 - Prob. 1AYLOCh. 18.5 - Prob. 2AYLOCh. 18.5 - Prob. 3AYLOCh. 18.5 - Prob. 4AYLOCh. 18.5 - Prob. 5AYLOCh. 18.5 - Prob. 6AYLOCh. 18.5 - Prob. 7AYLOCh. 18.5 - Prob. 8AYLOCh. 18.5 - Prob. 9AYLOCh. 18.5 - Prob. 10AYLOCh. 18.5 - Prob. 11AYLOCh. 18.5 - Terms for unwanted or inappropriate clotting in a...Ch. 18.5 - Prob. 13AYLOCh. 18 - Antibodies belong to a class of plasma proteins...Ch. 18 - Prob. 2TYRCh. 18 - Prob. 3TYRCh. 18 - Prob. 4TYRCh. 18 - Prob. 5TYRCh. 18 - Prob. 6TYRCh. 18 - Prob. 7TYRCh. 18 - Prob. 8TYRCh. 18 - Prob. 9TYRCh. 18 - Prob. 10TYRCh. 18 - Prob. 11TYRCh. 18 - Prob. 12TYRCh. 18 - Prob. 13TYRCh. 18 - Prob. 14TYRCh. 18 - Prob. 15TYRCh. 18 - Prob. 16TYRCh. 18 - Prob. 17TYRCh. 18 - Prob. 18TYRCh. 18 - Prob. 19TYRCh. 18 - Prob. 20TYRCh. 18 - Prob. 1BYMVCh. 18 - Prob. 2BYMVCh. 18 - Prob. 3BYMVCh. 18 - Prob. 4BYMVCh. 18 - Prob. 5BYMVCh. 18 - Prob. 6BYMVCh. 18 - Prob. 7BYMVCh. 18 - Prob. 8BYMVCh. 18 - Prob. 9BYMVCh. 18 - Prob. 10BYMVCh. 18 - Prob. 1WWTSCh. 18 - Prob. 2WWTSCh. 18 - Prob. 3WWTSCh. 18 - Prob. 4WWTSCh. 18 - Prob. 5WWTSCh. 18 - Prob. 6WWTSCh. 18 - Prob. 7WWTSCh. 18 - All formed elements of the blood except platelets...Ch. 18 - When RBCs die and break down, the globin moiety of...Ch. 18 - Prob. 10WWTSCh. 18 - Prob. 1TYCCh. 18 - Prob. 2TYCCh. 18 - Prob. 3TYCCh. 18 - Prob. 4TYCCh. 18 - Prob. 5TYC
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